Cytauxzoon felis in Domestic Cats: A Molecular Study Using Real-Time PCR in Semnan, Iran.

IF 0.9 4区 医学 Q4 PARASITOLOGY
Masoud Ebrahimi Toodarvari, Hesamedin Eskafian, Hamid Staji, Mahmood Ahmadi-Hamedani
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引用次数: 0

Abstract

Background: Cytauxzoon felis is a protozoan parasite transmitted by ticks and affects felids. Acute infection in domestic cats is characterized by symptoms such as lethargy, anorexia, fever, and anemia.

Methods: The present study focuses on diagnosing and molecularly identifying C. felis using a real-time PCR method in cats from Semnan, Iran. During the winter and spring of 2024, two hundred cats were randomly selected from veterinary clinics in Semnan. Blood samples were collected from the cats for DNA extraction and molecular analysis. Samples were divided into 40 pooled of 5 samples, each consisting of a combination of 5 blood samples. Then, the genomic DNAs were extracted from blood specimens and screened by real-time PCR (RT-PCR) for the presence of C. felis infection by amplifying of ITS2 gene belonging to the Cytauxzoon genus.

Results: The results indicated that 6 out of the 200 blood samples were infected (3%).

Conclusion: This study was conducted for the first time in Semnan and shows that the prevalence of C. felis in cats is significant.

Abstract Image

Abstract Image

Abstract Image

伊朗Semnan地区家猫中猫科细胞繁殖体的实时荧光定量PCR分子研究。
背景:猫胞虫是一种由蜱传播并影响猫科动物的原生动物寄生虫。家猫急性感染的特点是嗜睡、厌食、发烧和贫血。方法:采用实时荧光定量PCR方法对伊朗塞姆南地区的猫进行诊断和分子鉴定。在2024年冬季和春季,从Semnan的兽医诊所随机选择了200只猫。从猫身上采集血液样本进行DNA提取和分子分析。样本分为40个池,每池5个样本,每个池由5个血液样本组合而成。然后,从血样中提取基因组dna,通过扩增属于Cytauxzoon属的ITS2基因,采用实时荧光定量PCR (RT-PCR)技术筛选是否存在猫卷虫感染。结果:200份血样中感染6份(3%)。结论:本研究为Semnan地区首次开展的研究,表明猫毛线虫在猫中的流行程度显著。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
55
审稿时长
6-12 weeks
期刊介绍: Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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