Enzyme inhibition-enabled CRISPR/Cas12a biosensing system for heparin-related non-nucleic acid biomarkers

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-07-24 DOI:10.1039/d5cc03376d

Ruo Ma, Wenjiao Fan, Yueran Wang, Xinrui Fei, Chenghui Liu

引用次数: 0

Abstract

Different from conventional CRISPR/Cas12a systems that must employ sophisticated functional nucleic acids, protein switch or allosteric transcription factors (aTFs)-based signal conversion for non-nucleic acid analysis, this work realizes more facile non-nucleic acid biomarker quantification through a new heparin-mediated Cas12a inhibition route.

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酶抑制CRISPR/Cas12a肝素相关非核酸生物标志物生物传感系统

与传统的CRISPR/Cas12a系统必须使用复杂的功能核酸、蛋白质开关或基于变steric转录因子（aTFs）的信号转换进行非核酸分析不同，本研究通过新的肝素介导的Cas12a抑制途径实现了更简便的非核酸生物标志物定量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.