Potential risks prompted from epicutaneous and intranasal exposure to indoxacarb in rats

IF 3.4 3区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicology and applied pharmacology Pub Date : 2025-07-18 DOI:10.1016/j.taap.2025.117479

Eman I. Hassanen , Marwa H. Hassan , Ahmed M. Hussien

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引用次数: 0

Abstract

Indoxacarb (IDX) is a new highly selective insecticide with excellent activity against a wide variety of pests. This study evaluated the possibility of dermal and nasal mucosal absorption of indoxacarb in rats in order to assess the potential hazards to humans and animals. 28 female albino Wistar rats were randomly divided into 4 groups (n = 7) as follow: group 1 (Ctrl1) normal saline via intranasal route, group 2 (Ctrl2) normal saline via topical dressing, group 3 (IDX-IN) IDX via intranasal route (0.42 mg/L, 1/10 LC50), and group 4 (IDX-T) the same concentration of IDX via topical dressing. The results revealed the highest serum levels of ALT, AST, CPK, and LDH in the IDX-T group, but the lowest level of IgG and IgM were recorded in the IDX-IN group. Furthermore, the IDX-T group exhibited various degrees of histopathological changes in most organs including skin, liver, heart, and lungs, while IDX-IN group showed prominent lesions in the lungs, immune organs, and most brain areas. The histopathological lesions were confirmed by the caspase-3 immunostaining that demonstrated strong casp-3 expression in the lungs, spleen, thymus, and brain of IDX-IN group, whereas IDX-T group noticed higher casp-3 expression in liver, heart, and lungs sections than IDX-IN group.

查看原文本刊更多论文

大鼠皮肤和鼻内接触吲哚虫威的潜在风险。

茚虫威（IDX）是一种新型的高选择性杀虫剂，对多种害虫具有优良的杀灭活性。本研究评估了茚虫威在大鼠皮肤和鼻黏膜吸收的可能性，以评估其对人类和动物的潜在危害。雌性白化Wistar大鼠28只，随机分为4组（n = 7）：1组（Ctrl1）鼻内生理盐水，2组（Ctrl2）生理盐水外用敷料，3组（IDX- in） IDX鼻内（0.42 mg/L, 1/10 LC50）， 4组（IDX- t）相同浓度的IDX外用敷料。结果显示，血清ALT、AST、CPK、LDH水平均以IDX-T组最高，IgG、IgM水平以IDX-IN组最低。此外，IDX-T组在皮肤、肝脏、心脏、肺等大部分器官均出现不同程度的组织病理学改变，而IDX-IN组在肺、免疫器官和大部分脑区均出现明显病变。组织病理学病变通过caspase-3免疫染色证实，IDX-IN组肺、脾、胸腺和脑组织中casp-3表达较强，而IDX-T组肝脏、心脏和肺组织中casp-3表达高于IDX-IN组。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology and applied pharmacology 医学-毒理学

CiteScore

6.80

自引率

2.60%

发文量

309

审稿时长

32 days

期刊介绍： Toxicology and Applied Pharmacology publishes original scientific research of relevance to animals or humans pertaining to the action of chemicals, drugs, or chemically-defined natural products. Regular articles address mechanistic approaches to physiological, pharmacologic, biochemical, cellular, or molecular understanding of toxicologic/pathologic lesions and to methods used to describe these responses. Safety Science articles address outstanding state-of-the-art preclinical and human translational characterization of drug and chemical safety employing cutting-edge science. Highly significant Regulatory Safety Science articles will also be considered in this category. Papers concerned with alternatives to the use of experimental animals are encouraged. Short articles report on high impact studies of broad interest to readers of TAAP that would benefit from rapid publication. These articles should contain no more than a combined total of four figures and tables. Authors should include in their cover letter the justification for consideration of their manuscript as a short article.