Plasma chylomicron-rich lipidomic and fatty acid profile following a meat or analogue meal in healthy males: a randomized controlled trial.

IF 6.5 1区医学 Q1 NUTRITION & DIETETICS

American Journal of Clinical Nutrition Pub Date : 2025-07-15 DOI:10.1016/j.ajcnut.2025.07.010

Toan Pham, Lillian Morton, Nicola Gillies, Emma Bermingham, Scott Knowles, David Cameron-Smith, Andrea J Braakhuis

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引用次数: 0

Abstract

Background: The composition of a meal impacts the digestion and absorption of dietary fats, potentially affecting their role in cardiovascular and chronic disease risk.

Objective: To compare the fatty acid concentrations, including LCω3FA, the lipidome and lipids in response to a meal containing grain-fed beef, pasture-fed beef, lamb or Beyond Burger (PBMA).

Methods: We conducted a randomized crossover trial where healthy males (n = 27) consumed a standardized burrito-style meal containing meat or alternative matched for protein and carbohydrate content on four separate occasions, with a minimum washout period of 1-week. Blood samples were collected before the meal and hourly for 4 hours after the meal. The changes in the fatty acid distribution, the lipidome of the chylomicron rich fraction (CMRF) and plasma lipids were analyzed.

Results: Linear mixed modelling and mixed effect ANOVA showed significant changes in the fatty acid postprandial response following all meals (p < 0.05) and subtle differences between meal types. Significant lipidomic features were predominantly TG for time (89 of 223) and meal (63 of 66). Meat meals had higher lysophosphatidylcholines in the chylomicrons (χ²(3) = 6.00, p= .042; W = 0.074, 95% CI 0.024, 1.030). The grain beef and lamb showed a significantly lower triglyceride response in plasma compared to PBMA (grain: -0.346, 95% CI -0.692 to - 0.0004; lamb: (-0.458, 95% CI -0.880 to -0.034).

Conclusions: There are subtle differences in the lipid response to beef, lamb and PMBA containing meal. This trial was registered at ClinicalTrials.gov as NCT04545398. https://clinicaltrials.gov/study/NCT04545398.

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健康男性肉类或类似物餐后血浆乳糜微粒富脂组学和脂肪酸谱：一项随机对照试验

背景：膳食的组成影响膳食脂肪的消化和吸收，潜在地影响它们在心血管和慢性疾病风险中的作用。目的：比较谷物饲养的牛肉、牧场饲养的牛肉、羊肉和Beyond Burger （PBMA）的脂肪酸浓度，包括LCω3FA、脂质组和脂质。方法：我们进行了一项随机交叉试验，其中健康男性（n = 27）在四个不同的场合食用含有肉类或蛋白质和碳水化合物含量匹配的替代品的标准化卷饼式膳食，最小洗脱期为1周。餐前和餐后4小时每小时采集一次血样。分析了脂肪酸分布、富乳糜微粒组分（CMRF）脂质组及血浆脂质的变化。结果：线性混合模型和混合效应方差分析显示，所有膳食后脂肪酸的餐后反应变化显著（p < 0.05），膳食类型之间存在细微差异。显著的脂质组学特征主要是时间TG（223人中有89人）和膳食TG（66人中有63人）。肉粉中乳糜微粒溶血磷脂酰胆碱含量较高(χ2(3) = 6.00, p= 0.042；W = 0.074, 95% ci 0.024, 1.030)。谷物牛肉和羊肉的血浆甘油三酯反应明显低于PBMA(谷物：-0.346,95% CI -0.692至- 0.0004；羔羊：（-0.458,95% CI -0.880至-0.034）。结论：牛肉、羊肉和含PMBA的饲料对脂肪的反应存在细微差异。该试验在ClinicalTrials.gov注册为NCT04545398。https://clinicaltrials.gov/study/NCT04545398。

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来源期刊

American Journal of Clinical Nutrition 医学-营养学

CiteScore

12.40

自引率

4.20%

发文量

332

审稿时长

38 days

期刊介绍： American Journal of Clinical Nutrition is recognized as the most highly rated peer-reviewed, primary research journal in nutrition and dietetics.It focuses on publishing the latest research on various topics in nutrition, including but not limited to obesity, vitamins and minerals, nutrition and disease, and energy metabolism. Purpose: The purpose of AJCN is to: Publish original research studies relevant to human and clinical nutrition. Consider well-controlled clinical studies describing scientific mechanisms, efficacy, and safety of dietary interventions in the context of disease prevention or health benefits. Encourage public health and epidemiologic studies relevant to human nutrition. Promote innovative investigations of nutritional questions employing epigenetic, genomic, proteomic, and metabolomic approaches. Include solicited editorials, book reviews, solicited or unsolicited review articles, invited controversy position papers, and letters to the Editor related to prior AJCN articles. Peer Review Process: All submitted material with scientific content undergoes peer review by the Editors or their designees before acceptance for publication.