scNanoHi-C2 uncovers dynamic reorganization of 3D chromatin structure in mammalian germ cells

Abstract

By leveraging long-read sequencing, single-cell input scNanoHi-C2 systematically delineates chromatin structural reprogramming in embryonic-stage germ cells (EGCs), highlighting the functional importance of three-dimensional (3D) genome structure in mammalian EGC development. This method provides a powerful tool for probing chromatin architecture in individual cells, particularly in rare or precious biological samples.