Zhaoqi Wang, Changping Xie, Xuetuan Lin, Li Zheng, Daipeng Chen
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Abstract
A previously uncharacterized mitovirus, designated as "Diaporthe mitovirus 3" (DMV3), was identified and isolated from the fungal pathogen Diaporthe sp. (isolate B-16) in this study. The DMV3 genome is a positive-sense, single-stranded RNA (+ ssRNA) molecule of 2,319 nucleotides (nt) with a GC content of 40%. It contains a single open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) consisting of 679 amino acids (aa) with a molecular mass of 76.4 kDa. Comparative analysis using BLASTp revealed that the RdRp of DMV3 exhibits the highest sequence similarity (64.56% identity) to Pestalotiopsis mitovirus 2 (PMV2). Phylogenetic analysis based on RdRp sequences demonstrated that DMV3 clusters together with PMV2 within a distinct clade, supporting its classification as a member of the genus Unuamitovirus within the family Mitoviridae.
期刊介绍：
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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