PEZO-1 is not required for AMsh glial responses to mechanical stimulation and does not play a major role in nose touch avoidance in C. elegans.

microPublication biology Pub Date : 2025-06-19 eCollection Date: 2025-01-01 DOI:10.17912/micropub.biology.001668
Jesus Fernandez-Abascal, Jesse D Hall, Laura Bianchi
{"title":"PEZO-1 is not required for AMsh glial responses to mechanical stimulation and does not play a major role in nose touch avoidance in <i>C. elegans</i>.","authors":"Jesus Fernandez-Abascal, Jesse D Hall, Laura Bianchi","doi":"10.17912/micropub.biology.001668","DOIUrl":null,"url":null,"abstract":"<p><p>In <i>Caenorhabditis elegans</i> , nose touch avoidance behavior is regulated by the Amphid Sheath (AMsh) glial cells via release of GABA. AMsh glia themselves exhibit calcium responses to mechanical stimulation, although the mechanosensitive channel responsible remains unidentified . Here, we investigated whether <i>pezo-1</i> , the sole <i>C. elegans</i> homolog of mammalian PIEZO1 and PIEZO2, mediates AMsh glial mechanosensitivity and contributes to nose touch avoidance. We examined behavioral responses in three <i>pezo-1</i> mutant strains, including a full gene deletion and two truncation alleles lacking the pore-forming transmembrane domains. In addition, we monitored calcium transients in the full gene deletion strain. While <i>pezo-1 ( av149 )</i> mutants showed a slight reduction in nose touch response, <i>pezo-1 ( av240 )</i> and <i>pezo-1 ( sy1199 )</i> mutants behaved like wild-type animals. In vivo calcium imaging revealed that AMsh glial responses to touch were preserved in <i>pezo-1 ( av240 )</i> mutants, with no significant difference in peak calcium signals compared to wild-type. These findings indicate that <i>pezo-1</i> is not required for AMsh glial mechanosensory responses or nose touch avoidance behavior. Further research is needed to identify the channels and pathways mediating mechanotransduction in AMsh glia.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231307/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001668","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

In Caenorhabditis elegans , nose touch avoidance behavior is regulated by the Amphid Sheath (AMsh) glial cells via release of GABA. AMsh glia themselves exhibit calcium responses to mechanical stimulation, although the mechanosensitive channel responsible remains unidentified . Here, we investigated whether pezo-1 , the sole C. elegans homolog of mammalian PIEZO1 and PIEZO2, mediates AMsh glial mechanosensitivity and contributes to nose touch avoidance. We examined behavioral responses in three pezo-1 mutant strains, including a full gene deletion and two truncation alleles lacking the pore-forming transmembrane domains. In addition, we monitored calcium transients in the full gene deletion strain. While pezo-1 ( av149 ) mutants showed a slight reduction in nose touch response, pezo-1 ( av240 ) and pezo-1 ( sy1199 ) mutants behaved like wild-type animals. In vivo calcium imaging revealed that AMsh glial responses to touch were preserved in pezo-1 ( av240 ) mutants, with no significant difference in peak calcium signals compared to wild-type. These findings indicate that pezo-1 is not required for AMsh glial mechanosensory responses or nose touch avoidance behavior. Further research is needed to identify the channels and pathways mediating mechanotransduction in AMsh glia.

在秀丽隐杆线虫中,AMsh胶质细胞对机械刺激的反应并不需要PEZO-1,并且在避免鼻子接触中也不起主要作用。
在秀丽隐杆线虫中,鼻触回避行为是由两栖鞘(AMsh)胶质细胞通过释放GABA来调节的。AMsh胶质细胞本身表现出钙对机械刺激的反应,尽管负责的机械敏感通道仍未确定。在这里,我们研究了PIEZO1和PIEZO2的唯一的线虫同源基因pezo-1是否介导AMsh胶质的机械敏感性,并有助于避免鼻子接触。我们研究了三个pezo-1突变株的行为反应,包括一个完整的基因缺失和两个缺乏形成孔的跨膜结构域的截断等位基因。此外,我们还监测了全基因缺失菌株的钙瞬变。pezo-1 (av149)突变体的鼻触反应略有降低,而pezo-1 (av240)和pezo-1 (sy1199)突变体的行为与野生型动物相似。体内钙成像显示,在pezo-1 (av240)突变体中,AMsh胶质细胞对触摸的反应被保留下来,与野生型相比,钙信号峰值没有显著差异。这些发现表明,pezo-1并不是AMsh神经胶质机械感觉反应或鼻触回避行为所必需的。需要进一步的研究来确定介导AMsh胶质细胞机械转导的通道和途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
审稿时长
3 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信