Genome structure and diversity of a novel ophio-like virus that infects the phytopathogenic fungus Rhizoctonia solani.

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-07-01 DOI:10.1007/s00705-025-06332-3

Yangyi Li, Xingxue Huang, Guolin Zhou, Anhua Ye, Yaohua Deng, Mengting Zhu, Haixiao Wang, Runhua Zhang

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引用次数: 0

Abstract

A new negative-stranded ophio-like RNA mycovirus, Rhizoctonia solani mycoophiovirus 1 (RsMOPV1), was isolated from strain JZ56 of Rhizoctonia solani AG-1 IA. The full-length RNA genome of RsMOPV1 is 7,317 nucleotides (nt) in length and consists of a single long open reading frame (ORF). The ORF encodes an RNA-dependent RNA polymerase (RdRp) consisting of 7,138 amino acids, with a predicted molecular mass of 273 kDa. The coding region of the RsMOPV1 genome is flanked by a short structured 3' untranslated region (3'-UTR) of 58 nucleotides and a 5' untranslated region (5'-UTR) of 121 nucleotides. We did not detect any additional segments of RsMOPV1. The nucleotide sequence of the genome of RsMOPV1 shares 74.7% identity with that of Rhizoctonia solani negative-stranded virus 2. RsMOPV1 infections may be associated with hypovirulence and a slow growth rate in R. solani. The RdRp of RsMOPV1 contains five typical RdRp motifs. Phylogenetic analysis demonstrated that RsMOPV1 clusters with members of the previously proposed genus "Mycoophiovirus".

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一种感染植物病原真菌枯丝核菌的新型蛇毒样病毒的基因组结构和多样性。

从solani Rhizoctonia AG-1 IA菌株JZ56中分离到一株新的负链蛇样RNA分枝病毒- solani mycoophhivirus 1 （RsMOPV1）。RsMOPV1全长RNA基因组长度为7317个核苷酸（nt），由一个长开放阅读框（ORF）组成。ORF编码一种RNA依赖的RNA聚合酶（RdRp），由7138个氨基酸组成，预测分子量为273 kDa。RsMOPV1基因组的编码区两侧是一个短结构的3‘非翻译区（3’-UTR），包含58个核苷酸和一个5‘非翻译区（5’-UTR），包含121个核苷酸。我们没有检测到任何额外的RsMOPV1片段。RsMOPV1与枯丝核负链病毒2的基因组核苷酸序列同源性为74.7%。RsMOPV1感染可能与索拉尼梭菌的低毒力和缓慢生长速率有关。RsMOPV1的RdRp包含5个典型的RdRp基序。系统发育分析表明，RsMOPV1病毒簇具有先前提出的“分枝杆菌病毒”属成员。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.