Hepatoprotective Activity of Red Ginger Extract on Acetaminophen-induced Liver Injury Models in HepG2 Cells.

IF 0.6 Q3 MEDICINE, GENERAL & INTERNAL

Annals of African Medicine Pub Date : 2025-06-30 DOI:10.4103/aam.aam_91_24

Wahyu Widowati, Hanna Sari Widya Kusuma, Didik Priyandoko, Wahyu Surakusumah, Nindia Salsabila Mia Dewi, Deni Rahmat, Dian Ratih Laksmitawati, Diah Kartika Pratami, Rizal Azis, Dhanar Septyawan Hadiprasetyo, Rita Tjokropranoto, Philips Onggowidjaja

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引用次数: 0

Abstract

Introduction: The liver is a major organ that plays a crucial role in detoxifying drugs; however, exposure to hepatotoxic drugs can lead to liver damage. Some research indicates that Zingiber officinale, commonly known as red ginger, possesses various biological properties. The objective of this study was to examine the hepatoprotective effect of red ginger extract (RGE) against acetaminophen (APAP)-induced HepG2 cells.

Materials and methods: HepG2 cells were induced with APAP to simulate liver injury and subsequently treated with RGE. Levels of nitric oxide (NO) and lactate dehydrogenase (LDH) were measured using a colorimetric assay, while the levels of interleukin (IL)-6, IL-1β, and IL-10 were measured using an enzyme-linked immunosorbent assay. Gene expression of caspase-3 (Casp-3), Casp-9, and c-Jun kinase (JNK) was assessed by quantitative real-time polymerase chain reaction.

Results: APAP-induced HepG2 cells exhibited a noticeable increase in pro-inflammatory markers. Cells treated with RGE showed lower levels of IL-1β and IL-6, and higher levels of IL-10 compared to the positive control. RGE treatment also reduced NO and LDH levels and downregulated the expression of Casp-3, Casp-9, and JNK genes.

Conclusions: These results indicate that RGE exerts hepatoprotective effects through increased IL-10 production, alongside the suppression of NO, IL-1β, IL-6, as well as Casp-3, Casp-9, and JNK gene expression.

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红姜提取物对对乙酰氨基酚所致HepG2细胞肝损伤模型的保护作用。

简介：肝脏是人体的重要器官，在解毒中起着至关重要的作用；然而，接触肝毒性药物会导致肝损伤。一些研究表明，红姜（Zingiber officinale）具有多种生物学特性。本研究旨在探讨红姜提取物（RGE）对对乙酰氨基酚（APAP）诱导的HepG2细胞的保护作用。材料和方法：用APAP诱导HepG2细胞模拟肝损伤，再用RGE处理。采用比色法测定一氧化氮（NO）和乳酸脱氢酶（LDH）水平，采用酶联免疫吸附法测定白细胞介素(IL)-6、IL-1β和IL-10水平。实时定量聚合酶链反应检测caspase-3 （Casp-3）、Casp-9和c-Jun激酶（JNK）的基因表达。结果：apap诱导的HepG2细胞显示出明显的促炎标志物升高。与阳性对照相比，经RGE处理的细胞IL-1β和IL-6水平较低，IL-10水平较高。RGE处理还降低了NO和LDH水平，下调了Casp-3、Casp-9和JNK基因的表达。结论：这些结果表明，RGE通过增加IL-10的产生，同时抑制NO、IL-1β、IL-6以及Casp-3、Casp-9和JNK基因的表达来发挥肝保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of African Medicine MEDICINE, GENERAL & INTERNAL-

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Annals of African Medicine is published by the Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria and the Annals of African Medicine Society. The Journal is intended to serve as a medium for the publication of research findings in the broad field of Medicine in Africa and other developing countries, and elsewhere which have relevance to Africa. It will serve as a source of information on the state of the art of Medicine in Africa, for continuing education for doctors in Africa and other developing countries, and also for the publication of meetings and conferences. The journal will publish articles I any field of Medicine and other fields which have relevance or implications for Medicine.