Advantages of three-photon live imaging for deep tissue analysis.

Expert review of medical devices Pub Date : 2025-06-27 DOI:10.1080/17434440.2025.2525497

Mary Ann Go, Mengke Yang, Simon R Schultz

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Abstract

Introduction: Three-photon microscopy is an emerging tool for deep tissue imaging with superior spatial resolution. It enables imaging of portions of tissue beyond the typical depth limit of two-photon microscopy.

Areas covered: In this review, we give an overview of widely used deep tissue imaging modalities. We highlight the advantages of three-photon microscopy and review its current applications in biomedical research as well as its potential pre-clinical and clinical applications. Finally, we assess the challenges in the widespread use of three-photon live imaging in biomedical research and in clinical translation.

Expert opinion: Three-photon microscopy holds great promise as a biomedical research tool. It can uncover insights into the structure and function of tissues beyond what is accessible with two-photon microscopy. It also has promise to be a powerful clinical tool. Future clinical applications include optical biopsies with near instantaneous results and guided surgical resections. A multimodal strategy that combines three-photon technology with a large field of view, low resolution technique may be used to overcome the limited field of view of three-photon microscopy for macroscopic tissue assessment.

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三光子实时成像在深部组织分析中的优势。

介绍：三光子显微镜是一种新兴的工具，深层组织成像具有优越的空间分辨率。它使组织的部分成像超出双光子显微镜的典型深度限制。涵盖的领域：在这篇综述中，我们给出了广泛使用的深部组织成像模式的概述。我们重点介绍了三光子显微镜的优点，并对其在生物医学研究中的应用现状以及临床前和临床应用前景进行了综述。最后，我们评估了在生物医学研究和临床翻译中广泛使用三光子实时成像的挑战。专家意见：三光子显微镜作为生物医学研究工具具有很大的前景。它可以揭示组织的结构和功能，而不是双光子显微镜所能达到的。它也有望成为一种强大的临床工具。未来的临床应用包括近乎即时结果的光学活检和引导手术切除。将三光子技术与大视场、低分辨率技术相结合的多模态技术可用于克服三光子显微镜宏观组织评估视场的局限性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Expert review of medical devices

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