Lucas Gruber, Idalia Soto, Tristan Correa, Esmeralda Apodaca, Brian Arreola, Jhamiley Cabral, Kristine Chen, Miguel Correo Galicia, Joanna Gomez, Ellaine Hao, Brisa Hernandez, Darien L Holland, Crystal Jara-Pichardo, Gwyniever M Lonzame, Abigail Ortiz Olguin, Rheana Romero, Devon Walker, Spencer G Amacher, Cooper Christie, Lillian G Coats, Aubrey Gerhardt, Claire A Holsted, Stephen R Kraizel, Ashley Krumlaw, Alex Stevens, Haleigh Stover, Chloe M Sullivan, Hailey Wyse, Danielle R Hamill, Jacob D Kagey, Kayla L Bieser
{"title":"The genetic mapping and phenotypic analysis of <i>Patronin <sup>F.1.1</sup></i> in <i>Drosophila melanogaster</i>.","authors":"Lucas Gruber, Idalia Soto, Tristan Correa, Esmeralda Apodaca, Brian Arreola, Jhamiley Cabral, Kristine Chen, Miguel Correo Galicia, Joanna Gomez, Ellaine Hao, Brisa Hernandez, Darien L Holland, Crystal Jara-Pichardo, Gwyniever M Lonzame, Abigail Ortiz Olguin, Rheana Romero, Devon Walker, Spencer G Amacher, Cooper Christie, Lillian G Coats, Aubrey Gerhardt, Claire A Holsted, Stephen R Kraizel, Ashley Krumlaw, Alex Stevens, Haleigh Stover, Chloe M Sullivan, Hailey Wyse, Danielle R Hamill, Jacob D Kagey, Kayla L Bieser","doi":"10.17912/micropub.biology.001564","DOIUrl":null,"url":null,"abstract":"<p><p>The multi-institutional Fly-CURE project is an undergraduate genetics research initiative centered on <i>Drosophila melanogaster</i> as a model organism. This study aimed to characterize and map mutations discovered through a Flp/FRT EMS screen to investigate complex interactions among genes associated with cell division, growth, and apoptosis leading to abnormal cell proliferation. The <i>F.1.1</i> mosaic phenotype resulted in a rough eye phenotype with an overall decrease in representation of mutant tissue. To genetically map the location of the <i>F.1.1</i> mutation, flies with genotype <i>FRT42D,F.1.1, Dark<sup>82</sup> /CyO</i> were crossed with the Bloomington 2R Deficiency Kit. The resultant F1 progeny were analyzed to pinpoint mapping deficiencies. The genomic region containing the <i>Patronin</i> gene was identified and sequencing confirmed the novel allele of <i>Patronin<sup>F.1.1</sup></i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186119/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001564","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The multi-institutional Fly-CURE project is an undergraduate genetics research initiative centered on Drosophila melanogaster as a model organism. This study aimed to characterize and map mutations discovered through a Flp/FRT EMS screen to investigate complex interactions among genes associated with cell division, growth, and apoptosis leading to abnormal cell proliferation. The F.1.1 mosaic phenotype resulted in a rough eye phenotype with an overall decrease in representation of mutant tissue. To genetically map the location of the F.1.1 mutation, flies with genotype FRT42D,F.1.1, Dark82 /CyO were crossed with the Bloomington 2R Deficiency Kit. The resultant F1 progeny were analyzed to pinpoint mapping deficiencies. The genomic region containing the Patronin gene was identified and sequencing confirmed the novel allele of PatroninF.1.1 .
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