Engineering saline-alkali-tolerant apple rootstock by knocking down MdGH3 genes in M9-T337.

Fang Zhi, Tianle Fan, Jia Li, Shuo Zhang, Qian Qian, Arij Khalil, Chundong Niu, Kun Wang, Fengwang Ma, Xuewei Li, Qingmei Guan
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Abstract

Soil salinization and alkalization have become an increasingly severe global issues, significantly limiting both the yield and quality of apples (Malus × domestica). M9-T337 is a widely used apple dwarfing rootstock; however, it is sensitive to saline-alkali stress. Therefore, developing saline-alkali tolerant apple rootstocks is essential. In this study, we utilized RNAi (RNA interference) technology to knock down GH3 genes in the M9-T337 background, aiming to engineer a dwarfing and stress-tolerant apple rootstock. We found that MdGH3 RNAi plants exhibited superior morphology compared to M9-T337 under saline-alkali stress conditions, characterized by more robust root systems, increased plant height, a lower Na+/K+ ratio, and enhanced photosynthetic and antioxidant capacities. Moreover, when MdGH3 RNAi plants were used as rootstocks, the GL-3/MdGH3 RNAi plants also displayed greater plant height, root vitality, photosynthetic ability, and antioxidant capacity compared to GL-3 grafted onto M9-T337 rootstock. Taken together, our study constructed a saline-alkali-tolerant apple rootstock by knocking down MdGH3 genes.

通过敲除M9-T337 MdGH3基因来改造耐盐碱苹果砧木。
土壤盐碱化已成为日益严重的全球性问题,严重制约了苹果(Malus × domestica)的产量和品质。M9-T337是一种应用广泛的苹果矮化砧木;但对盐碱胁迫较为敏感。因此,培育耐盐碱苹果砧木是十分必要的。在本研究中,我们利用RNAi (RNA干扰)技术敲低了M9-T337背景下的GH3基因,旨在设计一个矮化和耐胁迫的苹果砧木。我们发现,与M9-T337相比,MdGH3 RNAi植株在盐碱胁迫条件下表现出更优越的形态,根系更强健,株高增加,Na+/K+比更低,光合和抗氧化能力增强。此外,当MdGH3 RNAi植株作为砧木时,与嫁接到M9-T337砧木上的GL-3相比,GL-3/MdGH3 RNAi植株也表现出更高的株高、根系活力、光合能力和抗氧化能力。总之,我们的研究通过敲除MdGH3基因构建了一个耐盐碱的苹果砧木。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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