Generation of two novel furanosteroids via heterologous expression of demethoxyviridin biosynthetic genes in a wortmannin-producing strain†

IF 2.7 3区化学 Q1 CHEMISTRY, ORGANIC

Organic & Biomolecular Chemistry Pub Date : 2025-06-12 DOI:10.1039/d5ob00856e

Wei Jiang , Lin-Huan Li , Lin-Min Zhang , Huan Zhao , Shao-Yang Li , Gao-Qian Wang , Guo-Dong Chen , Jian-Ming Lv , Dan Hu , Hao Gao

引用次数: 0

Abstract

Furanosteroids, represented by wortmannin and demethoxyviridin, are a class of structurally unique steroids with significant bioactivities. In this study, wortmannin and its analogue 11-desacetoxywortmannin were isolated from Talaromyces wortmannii ATCC 26942. Subsequently, individual post-tailoring enzyme genes involved in the biosynthesis of demethoxyviridin were introduced into T. wortmannii ATCC 26942, leading to the detection of four putative novel furanosteroids. Among them, two compounds were isolated and identified, both of which are detrimental to Aspergillus niger R330 and Candida albicans FIM709.

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通过异源表达去甲氧基viridin生物合成基因在wortmannin生产菌株中产生两种新型呋喃甾体。

呋喃甾体是一类结构独特、具有显著生物活性的甾体，以wortmannin和demethoxyviridin为代表。本研究从Talaromyces wortmannii ATCC 26942中分离得到wortmannin及其类似物11-去乙酰氧基wortmannin。随后，将参与demethoxyviridin生物合成的单个后剪裁酶基因引入wortmannii ATCC 26942，从而检测到四种假定的新型呋喃甾体。其中分离鉴定出两种化合物，均对黑曲霉R330和白色念珠菌FIM709有害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organic & Biomolecular Chemistry 化学-有机化学

CiteScore

5.50

自引率

9.40%

发文量

1056

审稿时长

1.3 months

期刊介绍： Organic & Biomolecular Chemistry is an international journal using integrated research in chemistry-organic chemistry. Founded in 2003 by the Royal Society of Chemistry, the journal is published in Semimonthly issues and has been indexed by SCIE, a leading international database. The journal focuses on the key research and cutting-edge progress in the field of chemistry-organic chemistry, publishes and reports the research results in this field in a timely manner, and is committed to becoming a window and platform for rapid academic exchanges among peers in this field. The journal's impact factor in 2023 is 2.9, and its CiteScore is 5.5.