Analysis of mitochondrial membrane potential, ROS, and calcium

IF 6.5 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2025-06-16 DOI:10.1016/j.mocell.2025.100238

Hye-Kyung Park, Byoung Heon Kang

引用次数: 0

Abstract

Mitochondria play a central role in cellular energy metabolism and signaling, and their dysfunction is associated with a wide range of diseases. Therefore, assessing mitochondrial function is essential for understanding their role in various cellular processes and disease progression. Here, we describe the principles and methodologies for analyzing mitochondrial membrane potential, reactive oxygen species, and calcium levels using the fluorescent probes tetramethylrhodamine methyl ester, MitoSOX, and Rhod-2AM, respectively. This work provides a practical guide for researchers investigating mitochondrial function under physiological and pathological conditions.

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线粒体膜电位、活性氧和钙的分析。

线粒体在细胞能量代谢和信号传导中起着核心作用，其功能障碍与多种疾病有关。因此，评估线粒体功能对于了解它们在各种细胞过程和疾病进展中的作用至关重要。在这里，我们描述了使用荧光探针TMRM、MitoSOX和Rhod-2AM分别分析线粒体膜电位、活性氧和钙水平的原理和方法。这项工作为研究人员在生理和病理条件下研究线粒体功能提供了实用指南。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is "Mol. Cells". Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.