Molecular Characteristics and Phenotypical Analysis of Carbapenem-Resistant K. Pneumoniae in the Lüliang Region, Shanxi Province.

IF 2.9 3区医学 Q2 INFECTIOUS DISEASES

Infection and Drug Resistance Pub Date : 2025-06-07 eCollection Date: 2025-01-01 DOI:10.2147/IDR.S512203

Huiyuan Wang, Zijiang Jia, Xiaoyan Li, Yan Hao, Jinjing Zhang, Xinyi Zhao, Hui Li, Sifan Jin

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Abstract

Introduction: To explore the resistance characteristics and molecular features of carbapenem-resistant K. pneumoniae (CRKP) isolates prevalent in the Lüliang region, 81 CRKP isolates were collected from three hospitals in the Lüliang region, Shanxi Province.

Methods: The resistance of these CRKP isolates to 11 antibiotics was determined using the disk diffusion method, and antimicrobial resistance encoding genes and virulence genes were detected by PCR. The mucoid phenotype of the CRKP isolates was examined via the string test, and bacterial biofilm formation ability was measured using the crystal violet staining method.

Results: The resistance rates of the 81 CRKP isolates to the 11 antibiotics ranged from 62.96% to 100%, with a multidrug resistance rate of 83.95%. The resistance genes bla_SHV, bla_TEM and bla_KPC were the most widely distributed, with a detection rate of 100%. Among the 81 CRKP isolates, 70 had the ability to form biofilms, and 58 presented highly mucoid phenotypes. The virulence genes rmpA2, peg-344, and fimH presented high carriage rates of 92.59%, 91.36%, and 88.89%, respectively. The carriage rate of IroB was low, at 20.99%. Among these genes, fimH, rmpA2, and iucA were associated with biofilm formation, while markD and fimH were associated with a highly mucoid phenotype, and the highly mucoid phenotype was strongly correlated with the biofilm formation ability.

Conclusion: This study revealed that the CRKP strains isolated in the Lüliang region of Shanxi Province were strongly resistant and that this resistance was related to virulence characteristics. Therefore, antibiotic management should be strengthened in clinical practice to control the prevalence of CRKP in this region.

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山西省梁梁地区耐碳青霉烯类肺炎克雷伯菌分子特征及表型分析

前言：为探讨梁地区流行的耐碳青霉烯类肺炎克雷伯菌（CRKP）的耐药特征及分子特征，在山西省梁地区3家医院采集了81株CRKP分离株。方法：采用纸片扩散法检测分离株对11种抗生素的耐药性，并采用PCR检测耐药编码基因和毒力基因。采用串法检测菌株黏液表型，结晶紫染色法检测细菌生物膜形成能力。结果：81株CRKP对11种抗生素的耐药率为62.96% ~ 100%，其中多药耐药率为83.95%。耐药基因blaSHV、blaTEM和blaKPC分布最广，检出率为100%。在81株CRKP分离株中，70株具有形成生物膜的能力，58株具有高度黏液样表型。毒力基因rmpA2、peg-344和fih的携带率分别为92.59%、91.36%和88.89%。IroB的携带率较低，为20.99%。其中，fimH、rmpA2和iucA与生物膜形成相关，markD和fimH与高度黏液样表型相关，且高度黏液样表型与生物膜形成能力强相关。结论：山西梁梁地区分离的CRKP菌株具有较强的耐药性，且这种耐药性与毒力特性有关。因此，在临床实践中应加强抗生素管理，控制该地区CRKP的流行。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection and Drug Resistance Medicine-Pharmacology (medical)

CiteScore

5.60

自引率

7.70%

发文量

826

审稿时长

16 weeks

期刊介绍： About Journal Editors Peer Reviewers Articles Article Publishing Charges Aims and Scope Call For Papers ISSN: 1178-6973 Editor-in-Chief: Professor Suresh Antony An international, peer-reviewed, open access journal that focuses on the optimal treatment of infection (bacterial, fungal and viral) and the development and institution of preventative strategies to minimize the development and spread of resistance.