Visualizing chromatin communication in living cells through Oligo-LiveFISH.

Abstract

Understanding genome regulation requires capturing chromatin dynamics in real time. In a recent issue of Cell, Zhu et al. introduce Oligo-LiveFISH,¹ a high-resolution live-cell imaging platform that tracks non-repetitive loci without genome engineering. By assembling fluorescent guide RNA pools with dCas9, this method enables 20-nm, 50-ms resolution imaging, offering insights into dynamic genome organization across cell lines and primary cells.