Optogenetic neural spheroids excite primary neural network.

IF 3.8
Saeedur Rahman, Md Saddam Hossain Joy, M Taher A Saif
{"title":"Optogenetic neural spheroids excite primary neural network.","authors":"Saeedur Rahman, Md Saddam Hossain Joy, M Taher A Saif","doi":"10.1088/1741-2552/ade28d","DOIUrl":null,"url":null,"abstract":"<p><p><i>Objective.</i>Optical stimulation of<i>in vitro</i>neurons requires prior transfection with light gated ion channels. This additional step brings complexity and requires optimization. Simplification of the process will ease the undertaking of studies on biological neural networks needing external stimulation.<i>Approach.</i>We constructed a simple platform where embryonic stem cell derived optogenetic neural spheroids, cultured and maintained separately, can be seeded on top of the primary non-optogenetic neuron cultures.<i>Main results.</i>We found that the primary neural network can be stimulated through the spheroids. This allows making investigations like network response dynamics and pharmacological perturbations possible.<i>Significance.</i>Thus, our platform provides an on-demand method to stimulate neural preparations for many different studies.</p>","PeriodicalId":94096,"journal":{"name":"Journal of neural engineering","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of neural engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1088/1741-2552/ade28d","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Objective.Optical stimulation ofin vitroneurons requires prior transfection with light gated ion channels. This additional step brings complexity and requires optimization. Simplification of the process will ease the undertaking of studies on biological neural networks needing external stimulation.Approach.We constructed a simple platform where embryonic stem cell derived optogenetic neural spheroids, cultured and maintained separately, can be seeded on top of the primary non-optogenetic neuron cultures.Main results.We found that the primary neural network can be stimulated through the spheroids. This allows making investigations like network response dynamics and pharmacological perturbations possible.Significance.Thus, our platform provides an on-demand method to stimulate neural preparations for many different studies.

光遗传神经球激发初级神经网络。
目的:体外神经元的光刺激需要预先转染光门控离子通道。这个额外的步骤带来了复杂性,需要优化。该过程的简化将简化需要外部刺激的生物神经网络的研究工作。方法:我们构建了一个简单的平台,胚胎干细胞(ESC)衍生的光遗传神经球体,单独培养和维持,可以在原代非光遗传神经元培养物上播种。主要结果:我们发现初级神经网络可以通过球体来刺激。这使得像网络反应动力学和药理学扰动这样的研究成为可能。意义:因此,我们的平台为许多不同的研究提供了一种按需刺激神经准备的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信