Design and application of hydroxyl ionic liquids for the supported synthesis of oligopeptides

Abstract

A strategy for the rapid and effective supported synthesis of oligopeptides using a novel hydroxyethyl imidazole-type ionic liquid was developed after systematically optimizing the ionic liquid structure depending on the separation and purification efficiency. This method, which incorporates the advantages of both solid- and liquid-phase synthesis, can not only maintain the homogeneous reaction conditions, but also facilitate the purification process with high product yield. The effectiveness of this approach was demonstrated by the successful synthesis of [Leu⁵]-enkephalin and [Met⁵]-enkephalin (yield ≥70 % and purity ≥95 %). The used ionic liquid support can be effectively recycled.