{"title":"<i>In vitro</i> CSLM evaluation of bonding agents in amalgam restoration replaced with composite resin.","authors":"Sayanti Ghosh, Pratima Shenoi, Manjeet Uke, Ankita Niswade","doi":"10.4103/JCDE.JCDE_165_25","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>This study aims to analyze the depth of penetration of two different adhesives using Confocal Laser Scanning Microscope in Class I amalgam restorations replaced with composite restorations.</p><p><strong>Subjects and methods: </strong>Eighty freshly extracted human premolars were selected for the study. Samples were randomly divided into four groups (<i>n</i> = 20). Class I cavities were prepared for composite resin restorations the adhesive used in Group Ia: Tetric N-Bond and Group Ib: Tetric N-Bond Universal. For Group II and Group III: Samples were first restored with silver amalgam and removed after 1 year followed by composite resin restoration: Adhesive used in Group II: Tetric N-Bond and Group III: Tetric N-Bond Universal. While using the adhesives each time, they were labeled with 0.1% Rhodamine B dye. After restoring the samples using Te-Econom Plus, they were sectioned with a microtome precision saw. CLSM was used to determine the thickness of the hybrid layer and the number of resin tags.</p><p><strong>Results: </strong>Higher values of thickness of hybrid layer and number of resin tags were observed in the control group as compared to the experimental groups.</p><p><strong>Conclusions: </strong>A significantly thicker hybrid layer and higher number of resin tags were obtained when a total-etch system was used for replacing old amalgam restoration with composite resin.</p>","PeriodicalId":516842,"journal":{"name":"Journal of conservative dentistry and endodontics","volume":"28 5","pages":"474-480"},"PeriodicalIF":0.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12129292/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of conservative dentistry and endodontics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/JCDE.JCDE_165_25","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/6 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Aim: This study aims to analyze the depth of penetration of two different adhesives using Confocal Laser Scanning Microscope in Class I amalgam restorations replaced with composite restorations.
Subjects and methods: Eighty freshly extracted human premolars were selected for the study. Samples were randomly divided into four groups (n = 20). Class I cavities were prepared for composite resin restorations the adhesive used in Group Ia: Tetric N-Bond and Group Ib: Tetric N-Bond Universal. For Group II and Group III: Samples were first restored with silver amalgam and removed after 1 year followed by composite resin restoration: Adhesive used in Group II: Tetric N-Bond and Group III: Tetric N-Bond Universal. While using the adhesives each time, they were labeled with 0.1% Rhodamine B dye. After restoring the samples using Te-Econom Plus, they were sectioned with a microtome precision saw. CLSM was used to determine the thickness of the hybrid layer and the number of resin tags.
Results: Higher values of thickness of hybrid layer and number of resin tags were observed in the control group as compared to the experimental groups.
Conclusions: A significantly thicker hybrid layer and higher number of resin tags were obtained when a total-etch system was used for replacing old amalgam restoration with composite resin.
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