Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4306-8_10

Feier Zeng, Silvia Marino, Aymen I Idris

引用次数: 0

Abstract

This chapter describes the analysis of signaling pathways in bone cells by the use of Western blotting and immunoprecipitation, including a step-by-step guide to cell culture techniques, cellular and subcellular fractionation, protein isolation, purification, measurement, electrophoretic transfer, and detection.

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Western Blotting和免疫沉淀分析信号通路。

本章描述了通过使用Western blotting和免疫沉淀对骨细胞信号通路的分析，包括细胞培养技术，细胞和亚细胞分离，蛋白质分离，纯化，测量，电泳转移和检测的逐步指南。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.