Genomic and phylogenetic analyses of the domoic acid biosynthesis genes in the benthic diatom Nitzschia navis-varingica (Bacillariophyceae)

IF 5.5 1区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Harmful Algae Pub Date : 2025-05-26 DOI:10.1016/j.hal.2025.102905

Zongmei Cui , Hui Wang , Po Teen Lim , Suh Nih Tan , Yuichi Kotaki , Chui Pin Leaw , Nansheng Chen

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Abstract

Through genome sequencing and comparative genomic analysis, candidate genes involved in the biosynthesis of domoic acid (DA) in the benthic diatom Nitzschia navis-varingica were identified. Orthologs of DA biosynthesis genes (dabA, dabB, dabC, and dabD), originally identified in Pseudo-nitzschia species, were detected in N. navis-varingica strains. However, due to the draft level of our genomes, the tandem gene cluster organization present in the Pseudo-nitzschia species cannot be determined in N. navis-varingica strains. In addition, two copies of dabC were found in N. navis-varingica strains, suggesting potential functional significance in the biosynthesis of DA and its isoforms. The identification of dab genes in N. navis-varingica provides a foundation for further studies on the origin, evolution, and transcriptional regulations of DA biosynthesis in this benthic diatom.

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底栖硅藻navis-varingica软骨藻酸生物合成基因的基因组和系统发育分析

通过基因组测序和比较基因组分析，确定了底栖硅藻耐藻（Nitzschia navis-varingica）软骨藻酸（domoic acid， DA）生物合成的候选基因。原在拟尼茨菌属中鉴定的DA生物合成基因（dabA、dabB、dabC和dabD）在navis-varingica菌株中检测到同源基因。然而，由于我们基因组的草图水平，伪尼茨氏菌种中存在的串联基因簇组织不能在navis-varingica菌株中确定。此外，在N. navis-varingica菌株中发现了两个dabC拷贝，提示其在DA及其亚型的生物合成中具有潜在的功能意义。naviis -varingica中dab基因的鉴定为进一步研究该底栖硅藻中DA生物合成的起源、进化和转录调控提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Harmful Algae 生物-海洋与淡水生物学

CiteScore

12.50

自引率

15.20%

发文量

122

审稿时长

7.5 months

期刊介绍： This journal provides a forum to promote knowledge of harmful microalgae and macroalgae, including cyanobacteria, as well as monitoring, management and control of these organisms.