Increased MPO concentration but decreased peroxidase activity in saliva of obese women.

IF 3.9 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Katarzyna Korybalska, Anna Surdacka, Anna Lehmann, Joanna Żuraszek-Szymańska, Janusz Witowski
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Abstract

Myeloperoxidase (MPO) is a key element of host defense against pathogens but is also implicated in disease. To explore whether MPO contributes to the postulated relationship between oral inflammation and obesity, the concentration of MPO and its activity were studied in saliva of obese and non-obese individuals. Whole mixed unstimulated saliva was obtained from 44 apparently healthy women without (n = 25) and with (n = 19) simple obesity. MPO concentration in saliva was measured by immunoassay; salivary peroxidation and chlorination activities were assessed with tetramethylbenzidine- and aminophenyl-fluorescein-based assays, respectively. Salivary MPO concentrations were found to be significantly higher in obese than in non-obese women and could distinguish these two populations. However, it did not correlate with total salivary peroxidation activity (TSPA), which was lower in obese women. MPO chlorinating activity was not detected in pure saliva in neither of the groups. The subjects' body weight, waist-to-hip ratio and the concentration of pro-inflammatory cytokines in saliva correlated positively with MPO concentration, but negatively with TSPA. In conclusion, MPO concentration in saliva is significantly increased in obesity but does not correlate with salivary peroxidation activity, which is lower in the obese. It may indicate that obesity impairs the ability of saliva to mitigate oxidative stress associated with inflammation.

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肥胖妇女唾液中MPO浓度升高,过氧化物酶活性降低。
髓过氧化物酶(MPO)是宿主防御病原体的关键因素,但也与疾病有关。为了探讨MPO是否与口腔炎症和肥胖之间的关系有关,我们研究了肥胖和非肥胖个体唾液中MPO的浓度及其活性。从44名无单纯性肥胖(n = 25)和单纯性肥胖(n = 19)的健康妇女获得了完整的混合未刺激唾液。免疫法测定唾液中MPO浓度;唾液过氧化和氯化活性分别以四甲基联苯胺和氨基苯基荧光素为基础进行测定。研究发现,肥胖女性的唾液MPO浓度明显高于非肥胖女性,这可以区分这两种人群。然而,它与总唾液过氧化活性(TSPA)无关,肥胖女性的TSPA较低。在两组的纯唾液中均未检测到MPO氯化活性。受试者体重、腰臀比、唾液促炎因子浓度与MPO浓度呈正相关,与TSPA呈负相关。综上所述,肥胖人群唾液中MPO浓度显著升高,但与唾液过氧化活性无关,肥胖人群唾液过氧化活性较低。这可能表明肥胖损害了唾液减轻与炎症相关的氧化应激的能力。
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来源期刊
Scientific Reports
Scientific Reports Natural Science Disciplines-
CiteScore
7.50
自引率
4.30%
发文量
19567
审稿时长
3.9 months
期刊介绍: We publish original research from all areas of the natural sciences, psychology, medicine and engineering. You can learn more about what we publish by browsing our specific scientific subject areas below or explore Scientific Reports by browsing all articles and collections. Scientific Reports has a 2-year impact factor: 4.380 (2021), and is the 6th most-cited journal in the world, with more than 540,000 citations in 2020 (Clarivate Analytics, 2021). •Engineering Engineering covers all aspects of engineering, technology, and applied science. It plays a crucial role in the development of technologies to address some of the world''s biggest challenges, helping to save lives and improve the way we live. •Physical sciences Physical sciences are those academic disciplines that aim to uncover the underlying laws of nature — often written in the language of mathematics. It is a collective term for areas of study including astronomy, chemistry, materials science and physics. •Earth and environmental sciences Earth and environmental sciences cover all aspects of Earth and planetary science and broadly encompass solid Earth processes, surface and atmospheric dynamics, Earth system history, climate and climate change, marine and freshwater systems, and ecology. It also considers the interactions between humans and these systems. •Biological sciences Biological sciences encompass all the divisions of natural sciences examining various aspects of vital processes. The concept includes anatomy, physiology, cell biology, biochemistry and biophysics, and covers all organisms from microorganisms, animals to plants. •Health sciences The health sciences study health, disease and healthcare. This field of study aims to develop knowledge, interventions and technology for use in healthcare to improve the treatment of patients.
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