Synthesis of Glycosylated 3-(3-Amino-3-carboxypropyl)uridine: A Minimum Unit of GlycoRNA.

Abstract

N-Glycosylated RNA (glycoRNA) has been identified on the cell surface, and 3-(3-amino-3-carboxypropyl)uridine has been reported as a conjugation site of N-glycans on RNA. Although the association of glycoRNAs with various diseases has been reported, their biosynthetic mechanisms and biological roles remain unexplored. Here, we report the preparation of two species of N-glycan-conjugated 3-(3-amino-3-carboxypropyl)uridine as the minimal units of glycoRNA. Our synthesized glycoRNA unit would contribute to future biochemical research on glycoRNAs.