Cystatin-mediated enhancement of human epidermal growth factor bioproduction in plants

IF 9.3 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Integrative Plant Biology Pub Date : 2025-05-28 DOI:10.1111/jipb.13936

Hyun Ji Park, Seung Hee Jo, Hyoenseo Park, Haemyeong Jung, Suk-Yoon Kwon, Hyun-Soon Kim, Sang Jik Kim, Hye Sun Cho

引用次数: 0

Abstract

Human epidermal growth factor (EGF) fused with the protease inhibitor SlCYS8 and produced in plants showed enhanced stability, yield, and biological activity. This fusion strategy reduced degradation and outperformed commercial EGF in cell-based assays, demonstrating a promising approach for producing stable, functional proteins in plant systems for pharmaceutical applications.

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半胱抑素介导的植物中人表皮生长因子生物生产的增强。

与蛋白酶抑制剂SlCYS8融合的人表皮生长因子（EGF）在植物中产生的稳定性、产量和生物活性均有所提高。这种融合策略减少了降解，并且在基于细胞的分析中优于商业EGF，证明了在植物系统中生产稳定的功能性蛋白质用于制药应用的有希望的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Integrative Plant Biology 生物-生化与分子生物学

CiteScore

18.00

自引率

5.30%

发文量

220

审稿时长

3 months

期刊介绍： Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.