Jérôme Trotereau, Anne-Sophie Huguet, Romain Jouan, Florent Kempf, Delphine Naquin, Catherine Schouler, Philippe Velge, Peter Mergaert, Isabelle Virlogeux-Payant
{"title":"Development and characterization of a saturated transposon mutant library of <i>Salmonella enterica</i> serovar Enteritidis LA5.","authors":"Jérôme Trotereau, Anne-Sophie Huguet, Romain Jouan, Florent Kempf, Delphine Naquin, Catherine Schouler, Philippe Velge, Peter Mergaert, Isabelle Virlogeux-Payant","doi":"10.1128/mra.01319-24","DOIUrl":null,"url":null,"abstract":"<p><p>We created and characterized a saturated Tn-seq mutant library in <i>Salmonella enterica</i> subsp. <i>enterica</i> ser. Enteritidis strain LA5. The set of essential genes after culture in rich medium was determined. This functional genomics tool will aid in identifying the roles of chromosomal and plasmid virulence determinants.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0131924"},"PeriodicalIF":0.7000,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12160557/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology Resource Announcements","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1128/mra.01319-24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/27 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
We created and characterized a saturated Tn-seq mutant library in Salmonella enterica subsp. enterica ser. Enteritidis strain LA5. The set of essential genes after culture in rich medium was determined. This functional genomics tool will aid in identifying the roles of chromosomal and plasmid virulence determinants.