Phylogeny of Aspergillus section Circumdati and inhibition of ochratoxins potential by green synthesised ZnO nanoparticles.

IF 4.4 2区生物学 Q1 MYCOLOGY

Mycology Pub Date : 2024-08-27 eCollection Date: 2025-01-01 DOI:10.1080/21501203.2024.2379480

Mohamed A Hussein, Youssuf A Gherbawy, Mahmoud S Abd El-Sadek, Helal F Al-Harthi, Eman Gam El-Dawy

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Abstract

Contamination of agricultural and industrial products by Aspergillus section Circumdati becomes a true problem, especially, because a lot of species in this section can yield ochratoxins and other toxins. In this study, morphological criteria and partial calmodulin gene were used to identify 34 strains belonging to Aspergillus section Circumdati isolated from Vitis vinifera and Calotropis procera plants. The population is characterised by A. insulicola, A. ochraceopetaliformis, A. ochraceus, and A. pseudoelegans. The polyketide synthase (pks) gene involved in ochratoxins (OTA) production was investigated by Aopks1, 2 and AoLc35-12L, R primers. Fifteen strains belonging to A. ochraceus were positive for pks gene, whilst A. insulicola, A. ochraceopetaliformis, and A. pseudoelegans were negative. All tested strains were able to produce ochratoxin A with different levels of 0.020-53 ppm except one isolate of A. ochraceus (IAEMAo6). The green synthesised ZnO-NPs have a significant inhibitory effect on OTA production by A. insulicola and A. pseudoelegans.

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绿色合成氧化锌纳米颗粒对圆形曲霉系统发育及赭曲霉毒素电位的抑制作用。

圆形曲霉对农业和工业产品的污染成为一个真正的问题，特别是因为该区域的许多种类可以产生赭曲霉毒素和其他毒素。本研究利用形态学标准和部分钙调蛋白基因对从葡萄和原卡罗普斯植物中分离的34株环曲霉进行了鉴定。种群的特征是岛蚜、长柄棘球绦虫、长柄棘球绦虫和假秀丽棘球绦虫。利用Aopks1, 2和AoLc35-12L， R引物对参与赭曲霉毒素（OTA）产生的聚酮合成酶（pks）基因进行了研究。15株猪棘球绦虫pks基因阳性，而猪棘球绦虫、牛棘球绦虫和假秀丽棘球绦虫pks基因阴性。除1株赭曲霉（IAEMAo6）外，所有菌株均能产生0.020 ~ 53 ppm的赭曲霉毒素A。绿色合成的ZnO-NPs对假秀丽隐杆线虫和假秀丽隐杆线虫的OTA产生有显著的抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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