3D In Vitro Culture of Early Mouse Embryos and Trophoblast Tissue Explants.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-05-22 DOI:10.1007/7651_2025_646

Devila Prit, Shashank Jaitly, Niraimathi Govindasamy, Adrian Ranga, Ivan Bedzhov

引用次数: 0

Abstract

The cellular dynamics during peri-implantation embryogenesis and the concurrent interactions at the embryo-maternal interface are inherently difficult to study due to intrauterine development in mammals. To model certain aspects of these processes in vitro, we have generated a biomimetic environment resembling the mechanical properties of the murine uterine stroma. Here we describe a step-by-step methodology for 3D culture of mouse embryos and ectoplacental cone explants in synthetic hydrogels that allow ex utero trophoblast invasion.

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小鼠早期胚胎和滋养细胞组织的3D体外培养。

由于哺乳动物的宫内发育，着床期胚胎发生过程中的细胞动力学以及胚胎-母体界面的同步相互作用本身就难以研究。为了在体外模拟这些过程的某些方面，我们已经产生了一个类似于小鼠子宫基质机械特性的仿生环境。在这里，我们描述了一个循序渐进的方法3D培养小鼠胚胎和胎盘外锥体外植体在合成水凝胶，允许体外滋养细胞入侵。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.