Correlation between MRNA IL1Β and type, duration of infertility in women with endometriosis on the stage preparing to assisted reproductive technologies using probiotics.

Q4 Medicine
Oksana V Bakun, Kristina V Dyak, Oksana V Kolesnik, Tetiana Protsak, Yevheniia A Dudka
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Abstract

Objective: Aim: To investigate the correlations between the level of IL1β mRNA gene expression and the type and duration of infertility and to study the levels of IL1β mRNA gene expression in women with endometriosis associated with infertility.

Patients and methods: Materials and Methods: For mRNA gene expression analysis IL1β and determination of relative normalized mRNA expression IL1β used the real-time reverse transcription polymerase chain reaction method (RT-PCR).. Examined group consists of 30 infertile women undergoing assisted reproductive technologies. The main group consisted of 20 women diagnosed with endometriosis undergoing assisted reproductive technologies. The control group consisted of 10 healthy women.

Results: Results: In the main group, the level of IL1β mRNA gene expression before preparing was 26.7877±0.01, which was significantly higher than the level after preparing (0.1610±0.01* ). Analized results, it's found out that Mean level of mRNA IL1β in women with endometriosis associated infertility 1-st degree is 8.53 c.u., at the same time Mean level of mRNA IL1β in women with endometriosis associated infertility 2-nd degree is 1.0 c.u.

Conclusion: Conclusions: The inclusion of probiotics in a comprehensive regimen of preparation for assisted reproductive technologies leads to a noticeable improvement in the patient's well-being.

MRNA IL1Β与子宫内膜异位症妇女在准备使用益生菌辅助生殖技术阶段的不孕症类型和持续时间的相关性
目的:探讨il - 1β mRNA基因表达水平与不孕症类型及持续时间的关系,探讨子宫内膜异位症合并不孕症患者il - 1β mRNA基因表达水平。患者和方法:材料和方法:采用实时逆转录聚合酶链反应(RT-PCR)法分析il - 1β mRNA基因表达,测定il - 1β mRNA相对标准化表达量。受检查组包括30名接受辅助生殖技术的不孕妇女。主要组由20名诊断为子宫内膜异位症的妇女组成,她们正在接受辅助生殖技术。对照组由10名健康女性组成。结果:结果:主组制备前il - 1β mRNA基因表达水平为26.7877±0.01,显著高于制备后水平(0.1610±0.01*);分析结果发现,子宫内膜异位症相关性不孕症1级患者的mRNA il - 1β平均水平为8.53 c.u,子宫内膜异位症相关性不孕症2级患者的mRNA il - 1β平均水平为1.0 c.u。结论:结论:在辅助生殖技术准备的综合方案中加入益生菌可显著改善患者的健康状况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Wiadomosci lekarskie
Wiadomosci lekarskie Medicine-Medicine (all)
CiteScore
0.80
自引率
0.00%
发文量
482
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