Development of an efficient extraction and enrichment method for total flavonoids compounds from Erigeron breviscapus using ultrasound-assisted extraction and macroporous resin adsorption.

IF 2 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

Preparative Biochemistry & Biotechnology Pub Date : 2025-05-15 DOI:10.1080/10826068.2025.2502757

Yang Li, Wei Zhao, Qifeng Li, Jiayu Zhang, Anquan Yang, Min Xie, Ranhua Xiong, Chaobo Huang

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Abstract

Erigeron breviscapus, a medicinal plant rich in flavonoids with diverse pharmacological properties is an important resource. To maximize the utilization of these valuable bioactive compounds, ultrasound-assisted extraction combined with response surface methodology was used to optimize the extraction conditions for total flavonoids. After comparing six macroporous resins' adsorption and desorption characteristics, AB-8 was selected for enriching total flavonoids, and its adsorption kinetics and thermodynamics were investigated. The following parameters were chosen as optimal for the dynamic adsorption/desorption experiments using an AB-8 filled column: sample loading concentration of 1.14 mg/mL, sample loading flow rate of 2 BV/h, elution solvent of 70% ethanol, desorption flow rate of 1 BV/h, elution solvent volume of 5.5 BV, pH of 2, temperature of 25 °C. After enrichment, the concentration of flavonoids was 5.16 times higher than that of crude extract. LC-MS identified 54 flavonoid molecules with excellent antioxidant properties.

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建立了超声辅助提取-大孔树脂吸附法提取灯盏花总黄酮的高效富集方法。

灯盏花（Erigeron breviscapus）是一种富含黄酮类化合物的药用植物，具有多种药理作用。为了最大限度地利用这些有价值的生物活性物质，采用超声辅助提取结合响应面法对总黄酮的提取条件进行了优化。通过比较6种大孔树脂的吸附和解吸特性，选择AB-8树脂富集总黄酮，并对其吸附动力学和热力学进行了研究。采用AB-8填充柱进行动态吸附/解吸实验的最佳参数为：上样浓度1.14 mg/mL，上样流速2 BV/h，洗脱溶剂70%乙醇，解吸流速1 BV/h，洗脱溶剂体积5.5 BV, pH = 2，温度25℃。富集后黄酮类化合物的含量是粗提物的5.16倍。LC-MS鉴定出54个具有优异抗氧化性能的类黄酮分子。

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来源期刊

Preparative Biochemistry & Biotechnology 工程技术-生化研究方法

CiteScore

4.90

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.