{"title":"Association between aquaporin 3 and transforming growth factor-beta 1 levels in decidual tissue and serum of patients with missed abortion.","authors":"JinLing Yuan, JianXin Dong, Wanting Li, YingMo Zu, YanJuan Guo, Ying Zhang, Yan Chen","doi":"10.3389/fmed.2025.1540257","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression of aquaporin 3 (AQP3) and transforming growth factor-β1 (TGF-β1) in the decidual tissue and serum of patients with missed abortion (MA) and explore their clinical significance, evaluating their potential as diagnostic biomarkers for MA.</p><p><strong>Methods: </strong>A total of 40 MA patients (case group) and 40 induced abortion (IA) patients (control group) were included. Immunohistochemistry (IHC), Western blot (WB), and reverse transcription quantitative PCR (RT-qPCR) were used to detect the protein and mRNA expression of AQP3 and TGF-β1 in decidual tissue. Serum levels of AQP3 and TGF-β1 were measured by ELISA. The diagnostic efficacy was assessed using receiver operating characteristic (ROC) curve analysis.</p><p><strong>Results: </strong>The protein expression of AQP3 and TGF-β1 in the decidual tissue of the MA group was significantly higher than that of the IA group, with a 2.3-fold and 2.5-fold increase, respectively (<i>p</i> < 0.01), and their mRNA expression was also significantly upregulated (<i>p</i> < 0.01). Serum levels of AQP3 and TGF-β1 increased by 2.3-fold and 3.3-fold, respectively (<i>p</i> < 0.01). ROC analysis demonstrated that serum AQP3 (AUC = 0.887) and TGF-β1 (AUC = 0.949) exhibited high diagnostic accuracy for MA, with the combined detection achieving an AUC of 0.976, sensitivity of 92.5%, and specificity of 97.5%.</p><p><strong>Conclusion: </strong>AQP3 and TGF-β1 are significantly overexpressed in the decidual tissue and serum of MA patients and may play a role in the pathogenesis of MA by regulating trophoblast function. The combined detection of these two biomarkers holds promise as potential diagnostic tools for MA, offering new directions for early clinical management.</p>","PeriodicalId":12488,"journal":{"name":"Frontiers in Medicine","volume":"12 ","pages":"1540257"},"PeriodicalIF":3.1000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12078160/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fmed.2025.1540257","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To investigate the expression of aquaporin 3 (AQP3) and transforming growth factor-β1 (TGF-β1) in the decidual tissue and serum of patients with missed abortion (MA) and explore their clinical significance, evaluating their potential as diagnostic biomarkers for MA.
Methods: A total of 40 MA patients (case group) and 40 induced abortion (IA) patients (control group) were included. Immunohistochemistry (IHC), Western blot (WB), and reverse transcription quantitative PCR (RT-qPCR) were used to detect the protein and mRNA expression of AQP3 and TGF-β1 in decidual tissue. Serum levels of AQP3 and TGF-β1 were measured by ELISA. The diagnostic efficacy was assessed using receiver operating characteristic (ROC) curve analysis.
Results: The protein expression of AQP3 and TGF-β1 in the decidual tissue of the MA group was significantly higher than that of the IA group, with a 2.3-fold and 2.5-fold increase, respectively (p < 0.01), and their mRNA expression was also significantly upregulated (p < 0.01). Serum levels of AQP3 and TGF-β1 increased by 2.3-fold and 3.3-fold, respectively (p < 0.01). ROC analysis demonstrated that serum AQP3 (AUC = 0.887) and TGF-β1 (AUC = 0.949) exhibited high diagnostic accuracy for MA, with the combined detection achieving an AUC of 0.976, sensitivity of 92.5%, and specificity of 97.5%.
Conclusion: AQP3 and TGF-β1 are significantly overexpressed in the decidual tissue and serum of MA patients and may play a role in the pathogenesis of MA by regulating trophoblast function. The combined detection of these two biomarkers holds promise as potential diagnostic tools for MA, offering new directions for early clinical management.
目的：研究水通道蛋白3 （AQP3）和转化生长因子-β1 （TGF-β1）在漏流产（MA）患者蜕膜组织和血清中的表达，探讨其临床意义，评价其作为漏流产（MA）诊断生物标志物的潜力。方法：选取40例MA患者（病例组）和40例人工流产（IA）患者（对照组）。采用免疫组化（IHC）、免疫印迹（WB）、反转录定量PCR （RT-qPCR）检测各组织中AQP3、TGF-β1蛋白及mRNA的表达情况。ELISA法检测血清AQP3、TGF-β1水平。采用受试者工作特征（ROC）曲线分析评价诊断效果。结果：MA组蜕膜组织中AQP3和TGF-β1蛋白表达明显高于IA组，分别升高2.3倍和2.5倍（p p p ）结论：AQP3和TGF-β1在MA患者蜕膜组织和血清中显著过表达，可能通过调节滋养细胞功能参与了MA的发病过程。这两种生物标志物的联合检测有望成为MA的潜在诊断工具，为早期临床管理提供新的方向。
期刊介绍：
Frontiers in Medicine publishes rigorously peer-reviewed research linking basic research to clinical practice and patient care, as well as translating scientific advances into new therapies and diagnostic tools. Led by an outstanding Editorial Board of international experts, this multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
In addition to papers that provide a link between basic research and clinical practice, a particular emphasis is given to studies that are directly relevant to patient care. In this spirit, the journal publishes the latest research results and medical knowledge that facilitate the translation of scientific advances into new therapies or diagnostic tools. The full listing of the Specialty Sections represented by Frontiers in Medicine is as listed below. As well as the established medical disciplines, Frontiers in Medicine is launching new sections that together will facilitate
- the use of patient-reported outcomes under real world conditions
- the exploitation of big data and the use of novel information and communication tools in the assessment of new medicines
- the scientific bases for guidelines and decisions from regulatory authorities
- access to medicinal products and medical devices worldwide
- addressing the grand health challenges around the world
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
