OT-Mation: an open-source code for parsing CSV files into Python scripts for control of OT-2 liquid-handling robotics.

IF 2.6 Q2 BIOCHEMICAL RESEARCH METHODS

Synthetic biology (Oxford, England) Pub Date : 2025-04-11 eCollection Date: 2025-01-01 DOI:10.1093/synbio/ysaf009

Alex Laverick, Katherine Convey, Catherine Harrison, Jenny Tomlinson, Jem Stach, Thomas P Howard

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Abstract

OT-Mation is an open-source Python script designed to automate the programming of OT-2 liquid-handling robots, making combinatorial experiments more accessible to researchers. By parsing user-defined CSV files containing information on labware, reagents, pipettes, and experimental design, OT-Mation generates a bespoke Python script compatible with the OT-2 system. OT-Mation enhances reproducibility, reduces human error, and streamlines workflows, making it a valuable addition to any laboratory utilizing OT-2 robotics for liquid handling. While OT-Mation can be used for setting up any type of experiment on the OT-2, its real utility lies in making the connection between multifactorial experimental design software outputs (i.e. design of experiments arrays) and liquid-handling robot executable code. As such, OT-Mation helps bridge the gap between code-based flexibility and user-friendly operation, allowing researchers with limited programming skills to design and execute complex experiments efficiently. Graphical Abstract.

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OT-Mation：用于将CSV文件解析为Python脚本以控制OT-2液体处理机器人的开源代码。

OT-Mation是一个开源的Python脚本，旨在自动化OT-2液体处理机器人的编程，使研究人员更容易进行组合实验。通过解析用户自定义的CSV文件，其中包含有关实验室仪器、试剂、移液器和实验设计的信息，OT-Mation生成与OT-2系统兼容的定制Python脚本。OT-Mation增强了再现性，减少了人为错误，简化了工作流程，使其成为任何使用OT-2机器人进行液体处理的实验室的宝贵补充。OT-Mation可以用于在OT-2上设置任何类型的实验，但其真正的用途在于将多因子实验设计软件输出（即实验阵列设计）与液体处理机器人可执行代码连接起来。因此，OT-Mation有助于弥合基于代码的灵活性和用户友好操作之间的差距，使编程技能有限的研究人员能够有效地设计和执行复杂的实验。图形抽象。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Synthetic biology (Oxford, England)

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