Maarten Besten, Milan Hendriksz, Lucile Michels, Bénédicte Charrier, Elwira Smakowska-Luzan, Dolf Weijers, Jan Willem Borst, Joris Sprakel
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引用次数: 0
Abstract
Plant cells are contained within a rigid network of cell walls. Cell walls serve as a structural material and a crucial signaling hub vital to all aspects of the plant life cycle. However, many features of the cell wall remain enigmatic, as it has been challenging to map its functional properties in live plants at subcellular resolution. Here, we introduce CarboTag, a modular toolbox for live functional imaging of plant walls. CarboTag uses a small molecular motif, a pyridine boronic acid, that directs its cargo to the cell wall. We designed a suite of cell wall imaging probes based on CarboTag in various colors for multiplexing. Additionally, we developed new functional reporters for live quantitative imaging of key cell wall characteristics: network porosity, cell wall pH and the presence of reactive oxygen species. CarboTag paves the way for dynamic and quantitative mapping of cell wall responses at subcellular resolution.
期刊介绍：
Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.
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