Multiplex PCR for the Rapid Diagnosis of Myeloproliferative Neoplasms.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-04-15 DOI:10.1007/7651_2025_625

Laura Semenuk, Alissa Palazzolo, Kristen McCambridge, Henry Wong, Michael J Rauh, Harriet Feilotter, Graeme Quest

引用次数: 0

Abstract

Myeloproliferative neoplasms (MPN) develop as a disease of aging, due to the disruption of normal hematopoiesis, triggered by somatic mutations and a sustained inflammatory cycle. Driving variants within JAK2, MPL, and CALR are associated with various subtypes of MPN, with known targeted therapeutics and guided patient prognosis. Targeted clinical testing for the diagnosis of these precise driving variants within these key genes has an integral role for personalized medicine.

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多重PCR快速诊断骨髓增生性肿瘤。

骨髓增生性肿瘤（MPN）是一种衰老疾病，由于正常造血功能的破坏，由体细胞突变和持续的炎症周期触发。JAK2、MPL和CALR中的驱动变异与MPN的各种亚型相关，具有已知的靶向治疗和指导患者预后。针对这些关键基因中这些精确驱动变异的诊断进行有针对性的临床测试，对于个性化医疗具有不可或缺的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.