Integrated meta-analysis, network pharmacology, computational biology, and in vitro experimental verification to reveal the anti-fatigue mechanism of Lycium barbarum.
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Abstract
This study aims to elucidate Lycium barbarum (LB)'s anti-fatigue mechanisms. Meta-analysis confirmed LB's anti-fatigue capacity, while network pharmacology, molecular docking, and molecular dynamics simulations identified key targets (SRC, HSP90AA1, EGFR, PRKACA, MAPK1). Furthermore, validation experiments in C2C12 cells demonstrated that LB mitigates H2O2-induced reactive oxygen species (ROS) accumulation and restores cell viability. qPCR analysis further revealed that LB downregulates the mRNA expression of CAT, IL-6 and TNF-α, while modulating the expression of these target genes. In summary, our data confirm the anti-fatigue effects of LB and elucidate that LB exerts multi-component, multi-target, and multi-pathway mechanisms in combating fatigue.
期刊介绍：
The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures.
All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.
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