Multifunctional au-DTNB-ag nanoparticles immunoprobes based on color-Raman properties for lateral flow immunoassay detection of Glycinin

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-05-15 DOI:10.1016/j.foodchem.2025.144788

Peng Bo Huang, Jing Long Qu, Shuo Yang, Tian Jiao Shui, Huan Huan Yang, Li Ping Cheng, Yang Qin, Jun Xi

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Abstract

Glycinin is a common food allergen, which may cause allergic shock in severe cases, thus posing a threat to public health. Therefore, rapid and accurate detection of glycinin is crucial. In this study, we synthesized AuAg core–shell nanoparticles loaded with the Raman reporter molecule 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), which provides both colorimetric and Raman signal capabilities. These nanoparticles were applied to colorimetric lateral flow immunoassay (CM-LFIA) and surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA), respectively. The CM-LFIA achieved a visual limit of detection (LOD) of 1 ng/mL, while the SERS-LFIA exhibited a LOD of 40 pg/mL, with good linearity over the range of 1 ng/mL to 10⁴ ng/mL (R² = 0.997). Moreover, the quantitative SERS-LFIA demonstrated satisfactory performance in spiked food matrix samples, achieving acceptable recoveries of 82–88 % and RSD ranging from 4.88 % to 6.97 %.

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基于彩色拉曼特性的多功能au-DTNB-ag纳米粒子免疫探针用于甘氨酸的横向流动免疫分析

甘氨酸是一种常见的食物过敏原，严重者可引起过敏性休克，对公众健康构成威胁。因此，快速准确地检测甘氨酸是至关重要的。在这项研究中，我们合成了装载拉曼报告分子5,5 ' -二硫代obis（2-硝基苯甲酸）（DTNB）的AuAg核壳纳米颗粒，该纳米颗粒同时具有比色和拉曼信号能力。这些纳米颗粒分别应用于比色横向流动免疫分析（CM-LFIA）和基于表面增强拉曼散射的横向流动免疫分析（SERS-LFIA）。CM-LFIA的检出限为1 ng/mL， SERS-LFIA的检出限为40 pg/mL，在1 ng/mL ~ 104 ng/mL范围内具有良好的线性关系（R2 = 0.997）。此外，定量SERS-LFIA在加标食品基质样品中表现出令人满意的性能，回收率为82 ~ 88 %，RSD范围为4.88 % ~ 6.97 %。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.