Isolation and characterization of a surfactin-like biosurfactant produced by Bacillus subtilis 3KP isolated from oil-contaminated soil in Indonesia.

IF 3.8 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience Reports Pub Date : 2025-04-29 DOI:10.1042/BSR20241227

Hanif Yuliani, Nimatuzahroh Nimatuzahroh, Handoko Darmokoesoemo, Hery Suwito, Eko Agus Nugroho, Moch Affandi, Agus Supriyanto, Fatimah Fatimah, Silvia Kurnia Sari, Ana Mariatul Khiftiyah

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引用次数: 0

Abstract

Biosurfactants are surface-active compounds that have many benefits. This research aims to isolate and identify biosurfactants produced by B. subtilis 3KP when cultured on a molasses substrate. The isolation and purification procedure included gradual ammonium sulfate precipitation, ion exchange and adsorption chromatography. Identification and characterization using high-pressure liquid chromatography, gas chromatography-mass spectrometry, amino acid analyzer, UV-V is spectroscopy, and infrared spectroscopy confirmed that B. subtilis 3KP biosurfactant is a lipopeptide. The biosurfactant produced by B. subtilis 3KP was determined to be a surfactin isoform, characterized by a 16-carbon fatty acid chain. The purified biosurfactant successfully decreased the surface tension from 72 mN/m to 27 mN/m and reached the critical micelle concentration at a precise value of 20.01 mg/L. This biosurfactant product is stable at 30°C-65°C, pH 4-8, and salinity 0-4 M. Therefore, the presented isolate has tremendous potential to produce surfactin-type biosurfactants which can be developed and utilized for various industrial fields.

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印尼石油污染土壤中枯草芽孢杆菌3KP类表面活性剂的分离与表征

生物表面活性剂是具有许多好处的表面活性化合物。本研究旨在分离和鉴定枯草芽孢杆菌3KP在糖蜜基质上培养时产生的生物表面活性剂。分离纯化工艺包括硫酸铵渐进沉淀法、离子交换法和吸附色谱法。采用高压液相色谱法、气相色谱-质谱法、氨基酸分析仪、紫外-紫外光谱法、红外光谱法对其进行鉴定和表征，证实枯草芽孢杆菌3KP生物表面活性剂为脂肽。由枯草芽孢杆菌3KP产生的生物表面活性剂为表面素异构体，其特征为16碳脂肪酸链。纯化后的生物表面活性剂成功地将表面张力从72 mN/m降低到27 mN/m，并达到临界胶束浓度20.01 mg/L。该生物表面活性剂产品在30°C ~ 65°C、pH 4 ~ 8、盐度0 ~ 4 m条件下具有较好的稳定性，因此该分离物具有巨大的生产表面素型生物表面活性剂的潜力，可开发利用于各种工业领域。

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来源期刊

Bioscience Reports 生物-细胞生物学

CiteScore

8.50

自引率

0.00%

发文量

380

审稿时长

6-12 weeks

期刊介绍： Bioscience Reports provides a home for sound scientific research in all areas of cell biology and molecular life sciences. Since 2012, Bioscience Reports has been fully Open Access and publishes all papers under the liberal CC BY licence, giving the life science community quality research to share and discuss.Content before 2012 is subscription-only, and is accessible via archive purchase. Articles are assessed on soundness, providing a home for valid findings and data. We welcome papers that span disciplines (e.g. chemistry, medicine), including papers describing: -new methodologies -tools and reagents to probe biological questions -mechanistic details -disease mechanisms -metabolic processes and their regulation -structure and function -bioenergetics