Recent advances in chemoenzymatic synthesis of human ABO blood group antigens

IF 12.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-05-11 DOI:10.1016/j.biotechadv.2025.108599

Jing Liu , Xianwei Liu , Wenyuan Fang

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引用次数: 0

Abstract

ABO blood group antigens (namely, A, B, and H antigens) are carbohydrate epitopes on cell surface that play important roles in biological processes and blood transfusion. The structure diversity of ABH antigens structures is associated with susceptibility to different pathogen infections. Advanced synthetic methodologies are required for studying and applying ABO blood group antigens. The existing methods include chemical, enzymatic and chemoenzymatic synthesis. As an alternative approach to chemical synthesis, enzymatic synthesis provides a simple pathway to access oligosaccharide antigens under extremely mild reaction conditions, thereby avoiding laborious protecting group manipulation procedures. Enzymatic synthesis of human blood group antigens primarily relies on Leloir glycosyltransferases, alongside several glycosidases and glycoside phosphorylases. This review aims to discuss recent advancements in chemical, enzymatic and chemoenzymatic synthesis of human ABH antigens, with a particular focus on novel developments in enzymatic assembly of naturally occurring ABH antigens.

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化学酶合成人ABO血型抗原的研究进展

ABO血型抗原（即A、B和H抗原）是细胞表面的碳水化合物表位，在生物过程和输血中起重要作用。ABH抗原结构的多样性与对不同病原体感染的易感性有关。ABO血型抗原的研究和应用需要先进的合成方法。现有的方法包括化学合成、酶合成和化学酶合成。作为化学合成的一种替代方法，酶合成提供了一种在极其温和的反应条件下获得低聚糖抗原的简单途径，从而避免了费力的保护基团操作程序。人类血型抗原的酶合成主要依赖于Leloir糖基转移酶，以及几种糖苷酶和糖苷磷酸化酶。这篇综述旨在讨论化学、酶和化学酶合成人类ABH抗原的最新进展，特别关注酶组装天然存在的ABH抗原的新进展。

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.