Cover Picture: Continuous Titration Based Method for Rapid In-Solution Analysis of Non-Covalent Interactions (Chem. Methods 5/2025)

Abstract

The Front Cover illustrates a new method (cSPRING) to study the interaction of a target molecule exposed to a continuous titration of a small ligand inside a flow capillary. The fluorescence emission of the target is detected in two separate spectral bands. A ratiometric measurement allows to extract the binding affinity (K_d) from a single measurement. Requiring only two samples and less than a minute, this method is well-suited for high-throughput screening applications. For more details, see the Research Article by Henrik Jensen and co-workers (10.1002/cmtd.202400059).