Improvement of semen quality in Longyou Partridge Chicken by dietary N-acetyl-L-glutamic acid and its mechanism study

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-04-30 DOI:10.1016/j.psj.2025.105234

Xue Du , Xiaopeng Tong , Xintong Qian , Yuanning Zhang , Duoxi Li , Xiaojun Liu , Xianzhong Zheng , Ayong Zhao

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引用次数: 0

Abstract

The aim of the study was to investigate the mechanisms of the N-acetyl-L-glutamic (NAG) diet in improving rooster semen quality after cryopreservation. A total of 60 individually housed chickens were randomly allocated to three groups: a basal diet group (C), a basal diet supplemented with 2 g/kg NAG group (NL), and a basal diet supplemented with 4 g/kg NAG group (NH). Semen quality was evaluated on days 15 and 30, including fresh sperm motility and cryopreservation efficacy. Serum biochemical parameters, hormone levels, immune indices, semen metabolites, and gut microbiota composition were assessed at 30 d. NAG supplementation significantly improved semen quality. At 15 and 30 d, both NL and NH groups showed a significant increase in the total motile sperm ratio (TM) in fresh semen compared to the control (P ≤ 0.05). At 30 d, group NH exhibited significantly higher percentage of sperm in a straight-line trajectory (LIN) in fresh semen than both the C and NL groups. After cryopreservation, the sperm TM and LIN from chickens with NAG dietary for 30 days were significantly better in the NL group compared to C and NH groups (P ≤ 0.05). Metabolomic analysis identified significant upregulation of metabolites, including melatonin and ferulic acid, in cryopreserved semen from NAG-supplemented groups, while 5-hydroxylysine and inosine-1-phosphate were significantly reduced. Serum antioxidant capacity, as measured by glutathione peroxidase (GSH-Px) was significantly higher, while malondialdehyde (MDA) levels lower in NAG groups compared to C (P ≤ 0.05). No significant differences in immune markers or reproductive hormones were observed across groups. Gut microbiota analysis revealed that NAG supplementation enhanced beneficial bacterial populations. In conclusion, NAG supplementation improved sperm motility and cryopreservation outcomes, potentially by increasing the levels of semen melatonin and ferulic acid and reducing the body oxidative stress. And it can improve the overall health level by regulating the gut microbiota through diet. These findings highlight NAG’s potential as a dietary supplement to improve semen quality and overall reproductive efficiency in poultry.

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n -乙酰- l-谷氨酸提高龙游鹧鸪鸡精液质量及其机理研究

本研究旨在探讨n -乙酰- l-谷氨酸（NAG）饲粮在提高公鸡冷冻后精液质量中的作用机制。试验选用60只单户饲养鸡，随机分为基础饲粮组(C)、基础饲粮中添加2 g/kg NAG组（NL）和基础饲粮中添加4 g/kg NAG组（NH）。在第15天和第30天评估精液质量，包括新鲜精子活力和冷冻保存效果。血清生化指标、激素水平、免疫指标、精液代谢物和肠道微生物群组成在30 d时进行了评估。15和30 d时，NL组和NH组鲜精液总活动精子比（TM）均显著高于对照组（P≤0.05）。30 d时，NH组新鲜精液中直线轨迹精子（LIN）百分比显著高于C组和NL组。低温保存后，饲粮添加NAG 30 d， NL组鸡精子TM和LIN显著优于C和NH组（P≤0.05）。代谢组学分析发现，在nag补充组冷冻保存的精液中，代谢物（包括褪黑激素和阿魏酸）显著上调，而5-羟赖氨酸和肌苷-1-磷酸显著降低。NAG组血清谷胱甘肽过氧化物酶（GSH-Px）测定抗氧化能力显著高于C组（P≤0.05），丙二醛（MDA）水平显著低于C组（P≤0.05）。各组间免疫标记物和生殖激素无显著差异。肠道菌群分析显示，NAG的补充增加了有益细菌的数量。综上所述，NAG的补充改善了精子活力和低温保存结果，可能是通过增加精液褪黑素和阿魏酸的水平以及减少身体氧化应激来实现的。通过饮食调节肠道菌群，提高整体健康水平。这些发现突出了NAG作为膳食补充剂在提高家禽精液质量和整体繁殖效率方面的潜力。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.