Quantification of Epigenetic DNA and RNA Modifications by UHPLC–MS/MS Technologies: New Concepts and New Improvements for the Special Collections

IF 2.8 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Journal of separation science Pub Date : 2025-05-08 DOI:10.1002/jssc.70159

Rui Zhang, Hailong Liu, Biao Bai, Hailin Wang

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Abstract

Dynamic and reversible DNA and RNA modifications are essential for cell differentiation and development. Aberrant epigenetic modifications are closely associated with the occurrence and progression of diseases, serving as potential markers for cancer diagnosis and prognosis. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) offers distinct advantages in the qualitative and quantitative analysis of various modifications due to its sensitivity, specificity, and accuracy. This review provides a comprehensive overview of the current knowledge regarding the liquid chromatography–mass spectrometry (LC–MS) analysis of DNA and RNA modifications, including analytical procedures, advancements, and biological applications, with a focus on tracing the source of (N6-2′-deoxy-adenosine) 6mdA in eukaryotes. Additionally, we examine the integration of UHPLC–MS/MS with other separation techniques to achieve accurate quantification of modifications in specific regions, certain fragments, and free nucleosides.

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UHPLC-MS /MS技术定量表观遗传DNA和RNA修饰：特殊馆藏的新概念和新改进

动态和可逆的DNA和RNA修饰是细胞分化和发育所必需的。异常的表观遗传修饰与疾病的发生和发展密切相关，是癌症诊断和预后的潜在标志。超高效液相色谱-串联质谱（UHPLC-MS /MS）由于其灵敏度、特异性和准确性，在各种修饰物的定性和定量分析中具有明显的优势。本文综述了目前关于DNA和RNA修饰的液相色谱-质谱（LC-MS）分析的知识，包括分析方法，进展和生物学应用，重点是追踪真核生物中（N6-2 ' -脱氧腺苷）6mdA的来源。此外，我们研究了UHPLC-MS /MS与其他分离技术的整合，以实现对特定区域、某些片段和游离核苷修饰的准确定量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of separation science 化学-分析化学

CiteScore

6.30

自引率

16.10%

发文量

408

审稿时长

1.8 months

期刊介绍： The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.