Improvement of cryopreserved epididymal ram sperm quality and fertility through curcumin nanoparticles

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-04-27 DOI:10.1016/j.theriogenology.2025.117462

Ramin Farhadi , Abbas Farshad , Abouzar Najafi , Jalal Rostamzadeh

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引用次数: 0

Abstract

Artificial insemination (AI) in rams relies on effective sperm preservation; however, freezing and thawing processes induce oxidative stress and sperm damage. This study evaluated the antioxidant effects of curcumin, in both free and nanoparticles (NPs) forms, on cryopreserved ram sperm. Curcumin was incorporated into the sperm extender at concentrations of 0, 25, and 50 μM. The curcumin NPs formulation, prepared using the nanoliposome technique, improved curcumin solubility and bioavailability. The 25 μM curcumin NPs group showed significantly higher sperm motility (total motility: 72.67 ± 1.15 %, progressive motility: 59.2 ± 0.96 %) compared to the control (total motility: 59.75 ± 0.68 %, progressive motility: 46.23 ± 1.50 %) (p < 0.05). Membrane (75.77 ± 0.87 %) and acrosome integrity (80.67 ± 0.87 %) were significantly improved in the 25 μM NPs group compared to those in the control (61.76 ± 0.97 %, 66.77 ± 1.50 %) (p < 0.05). Sperm viability was higher in the 25 μM NPs group (76.76 ± 1.22 %) vs. control (64.93 ± 0.80 %) (p < 0.05), and early apoptosis was reduced (5.06 ± 0.36 % vs. 9.7 ± 0.7 %) (p < 0.05). Oxidative stress markers were also improved, with lower MDA and ROS levels and enhanced antioxidant enzyme activity. The 25 μM curcumin NPs did not alter CYTb copy number. The 25 μM curcumin NPs group had higher fertility outcomes, including pregnancy (58.33 %), birth (58.33 %), and lambing (83.33 %) rates, than the control and 50 μM curcumin groups. These findings indicate that curcumin nanoparticles enhance sperm quality and fertility in cryopreserved ram sperm.

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姜黄素纳米颗粒改善附睾精子质量和生育能力

公羊人工授精依赖于有效的精子保存；然而，冷冻和解冻过程会引起氧化应激和精子损伤。本研究评估了游离和纳米颗粒形式的姜黄素（NPs）对冷冻保存的公羊精子的抗氧化作用。姜黄素以0、25和50 μM的浓度掺入精子扩展剂中。采用纳米脂质体技术制备的姜黄素NPs配方提高了姜黄素的溶解度和生物利用度。25 μM姜黄素NPs组精子活力（总活力：72.67±1.15%，进行性活力：59.2±0.96%）显著高于对照组（总活力：59.75±0.68%，进行性活力：46.23±1.50%）(p <；0.05)。与对照组（61.76±0.97%,66.77±1.50%）相比，25 μM NPs组膜（75.77±0.87%）和顶体完整性（80.67±0.87%）显著改善(p <；0.05)。25 μM NPs组精子存活率（76.76±1.22%）高于对照组（64.93±0.80%）(p <；0.05),和早期细胞凋亡减少(5.06±0.36%和9.7±0.7%)(p & lt;0.05)。氧化应激标志物也得到改善，MDA和ROS水平降低，抗氧化酶活性增强。25 μM姜黄素NPs未改变CYTb拷贝数。25 μM姜黄素NPs组的妊娠率（58.33%）、产仔率（58.33%）和产羔率（83.33%）均高于对照组和50 μM姜黄素组。这些发现表明，姜黄素纳米颗粒可以提高冷冻保存的公羊精子的精子质量和生育能力。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.