Factories without walls: The molecular architecture and functions of non-membrane organelles in small RNA-guided genome protection

IF 2.8 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. General subjects Pub Date : 2025-05-03 DOI:10.1016/j.bbagen.2025.130811

Shinichi Kawaguchi, Wakana Isshiki, Toshie Kai

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引用次数: 0

Abstract

Non-membrane organelles, Yb body and nuage, play an essential role in piRNA-guided genome defense in Drosophila gonad by mediating piRNA biogenesis and transposon silencing. Yb body, found in somatic follicle cells, is responsible for primary piRNA processing, while nuage, located in germline cells, facilitates the ping-pong cycle to amplify the piRNAs corresponding to both sense and antisense strands of the expressed transposons. These organelles are assembled by liquid-liquid phase separation (LLPS) and protein-protein interactions, integrating RNA helicases (Vasa, Armitage), Tudor domain-containing proteins (Krimper, Tejas, Qin/Kumo), and proteins containing both domains (Yb, SoYb, Spn-E). Within these condensates, we summarize the protein-protein interactions experimentally validated and predicted by AlphaFold3, providing new structural insights into the non-membrane organelle assembly. This review highlights how the dynamic organization of Yb body and nuage enables efficient RNA processing, ensuring transposon suppression and genome stability.

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无壁工厂：小rna引导的基因组保护中非膜细胞器的分子结构和功能

非膜细胞器Yb体和nuage通过介导piRNA生物发生和转座子沉默，在piRNA引导的果蝇性腺基因组防御中发挥重要作用。在体细胞卵泡细胞中发现的Yb小体负责初级piRNA加工，而位于种系细胞中的nuage则促进乒乓循环，扩增与表达转座子的正义链和反义链相对应的piRNA。这些细胞器通过液-液相分离（LLPS）和蛋白-蛋白相互作用组装，整合RNA解旋酶（Vasa, Armitage）， Tudor结构域蛋白（Krimper, Tejas, Qin/Kumo）以及包含两个结构域的蛋白（Yb, SoYb, Spn-E）。在这些凝聚物中，我们总结了通过AlphaFold3实验验证和预测的蛋白质-蛋白质相互作用，为非膜细胞器组装提供了新的结构见解。这篇综述强调了Yb体和细胞核的动态组织如何实现有效的RNA加工，确保转座子抑制和基因组稳定性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochimica et biophysica acta. General subjects 生物-生化与分子生物学

CiteScore

6.40

自引率

0.00%

发文量

139

审稿时长

30 days

期刊介绍： BBA General Subjects accepts for submission either original, hypothesis-driven studies or reviews covering subjects in biochemistry and biophysics that are considered to have general interest for a wide audience. Manuscripts with interdisciplinary approaches are especially encouraged.