Anti-inflammatory effects of apigenin on LPS-induced mastitis in lactating SD rats through inhibiting TLR4/NF-κB signaling pathway

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2025-04-26 DOI:10.1016/j.cyto.2025.156944

Yihan Hu , Yingying Xie , Yiming Sun , Linghuan Luo , Haibin Wang , Ruili Zhang , Ming Ge

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引用次数: 0

Abstract

Mastitis is an important disease of the mammary gland in all kinds of lactating mammals, endangering the development of animal husbandry and human health. Apigenin is one chemical constituent of Taraxacum and Philippine Violet Herb which are effective Chinese herbs for the treatment of mastitis. It is reported that apigenin possesses anti-inflammatory activity and other pharmacological effects. However, the attenuation of apigenin on mastitis has not yet been reported. The present study investigated the protection of apigenin against lipopolysaccharide (LPS)-induced mastitis in SD rats both in vivo and in vitro. The results suggested that apigenin relieved the lesions of mammary tissues induced by LPS, decreased mRNA and protein levels of pro-inflammatory cytokines:tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). Simultaneously, apigenin reduced the increasing content of myeloperoxidase (MPO) and Toll-like receptor 4 (TLR4), and phosphorylation of nuclear factor kappa B (NF-κB) induced by LPS. The results showed that apigenin was able to attenuate the LPS-induced mastitis in rats by inhibiting the TLR4/NF-κB signaling pathway in vivo and in vitro, which provides scientific references for further research.

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芹菜素通过抑制TLR4/NF-κB信号通路对哺乳期SD大鼠lps诱导的乳腺炎的抗炎作用

乳腺炎是各种哺乳期哺乳动物乳腺的重要疾病，危害着畜牧业的发展和人类的健康。芹菜素是一种化学成分的蒲公英和菲律宾堇菜是有效的中药治疗乳腺炎。据报道，芹菜素具有抗炎活性和其他药理作用。然而，芹菜素对乳腺炎的抑制作用尚未见报道。本研究在体内和体外研究了芹菜素对脂多糖（LPS）诱导的SD大鼠乳腺炎的保护作用。结果提示，芹菜素可减轻LPS诱导的乳腺组织病变，降低促炎因子肿瘤坏死因子-α (TNF-α)、白细胞介素-1β (IL-1β)和白细胞介素-6 (IL-6) mRNA和蛋白水平。同时，芹菜素降低了LPS诱导的髓过氧化物酶（MPO）和toll样受体4 （TLR4）含量升高以及核因子κB （NF-κB）磷酸化水平。结果表明，芹菜素在体内和体外均可通过抑制TLR4/NF-κB信号通路减轻lps诱导的大鼠乳腺炎，为进一步研究提供科学参考。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.