Virus-induced genome editing using a miniature CRISPR system

IF 15.8 1区生物学 Q1 PLANT SCIENCES

Nature Plants Pub Date : 2025-04-23 DOI:10.1038/s41477-025-01990-2

引用次数: 0

Abstract

The tobacco rattle virus was engineered to express the RNA-guided TnpB enzyme ISYmu1 and guide RNA. This miniature CRISPR system enabled transgene-free germline genome editing in Arabidopsis thaliana without the need for tissue culture or plant transformation.

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利用微型CRISPR系统进行病毒诱导的基因组编辑

设计烟草摇铃病毒，表达RNA引导的TnpB酶ISYmu1和引导RNA。这种微型CRISPR系统在不需要组织培养或植物转化的情况下，使拟南芥的无转基因生殖系基因组编辑成为可能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Plants PLANT SCIENCES-

CiteScore

25.30

自引率

2.20%

发文量

196

期刊介绍： Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.