Expression of size-selected messenger RNA encoding the brain and adrenal gland angiotensin-II receptor in Xenopus laevis oocytes.

N C Inestrosa, D Cross
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Abstract

The expression of the rat angiotensin-II receptor has been studied in Xenopus oocytes. Poly(A)+ RNA isolated from the brain and adrenal gland was injected into oocytes, and the expression of the receptor in the oocyte plasma membrane was assayed by measuring the change in membrane potential in the presence of angiotensin-II. Expression of the angiotensin-II receptor was detected 1.0-1.5 days after messenger RNA (mRNA) injection, and the degree of membrane depolarization was proportional to the amount of mRNA injected. Ca+2 channel blockers inhibited the angiotensin-II-induced depolarization. The total mRNA was fractionated by preparative agarose gel electrophoresis and each fraction was assayed for its ability to induce angiotensin-II depolarization. The mRNA encoding the angiotensin-II receptor was found in a single fraction of 4.4 kilobases.

编码脑和肾上腺血管紧张素ii受体的大小选择信使RNA在非洲爪蟾卵母细胞中的表达。
研究了大鼠血管紧张素- ii受体在非洲爪蟾卵母细胞中的表达。将脑和肾上腺分离的Poly(A)+ RNA注射到卵母细胞中,通过测量血管紧张素- ii存在时膜电位的变化来检测该受体在卵母细胞质膜中的表达。注射信使RNA (mRNA)后1.0 ~ 1.5 d检测血管紧张素- ii受体的表达,膜去极化程度与mRNA的注射量成正比。Ca+2通道阻滞剂抑制血管紧张素ii诱导的去极化。制备琼脂糖凝胶电泳对总mRNA进行分离,测定各部分诱导血管紧张素- ii去极化的能力。在4.4千碱基的单个片段中发现了编码血管紧张素- ii受体的mRNA。
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