HPLC–MS/MS Methods for Detecting Muscarine, Muscimol, and Ibotenic Acid in Fungal Samples and Biological Matrices

IF 1 4区化学 Q4 CHEMISTRY, ANALYTICAL

Journal of Analytical Chemistry Pub Date : 2025-04-22 DOI:10.1134/S1061934824701715

M. Sh. Aigumov, A. P. Novikov, M. V. Vishnevskii, N. A. Chernova, I. V. Maidanets, M. A. Gofenberg, D. V. Kuznetsov, N. V. Samyshkina, L. N. Rizvanova, A. Z. Temerdashev, S. A. Savchuk

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Abstract

This paper describes a two-step algorithm for detecting muscarine, ibotenic acid, and muscimol in urine samples. A two-step procedure was developed for detecting muscarine, muscimol, and ibotenic acid using a data-dependent mode of MRM analysis with the simultaneous full mass spectrum recording of the target substances. Analytes were detected by HPLC coupled with a triple quadrupole mass spectrometric detector (LC–MS/MS Shimadzu 8050). Chromatographic separation was performed in a Phenomenex Kinetex C18 reversed-phase column. At the first step, muscarine was detected in a diluted urine sample. At the second step, muscimol and ibotenic acid were found using a bimolecular dansylation method. The procedure has been successfully applied to routine chemical and toxicological analyses of urine samples from patients suspected of mushroom poisoning.

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HPLC-MS /MS检测真菌样品和生物基质中Muscarine、Muscimol和Ibotenic酸的方法

本文描述了一种检测尿样中毒蕈碱、伊博藤酸和毒蕈醇的两步算法。采用数据依赖的MRM分析模式，同时对目标物质进行全质谱记录，开发了两步程序，用于检测毒蕈碱、muscimol和伊博滕酸。采用高效液相色谱联用三重四极杆质谱检测器（LC-MS /MS Shimadzu 8050）进行分析。采用Phenomenex Kinetex C18反相色谱柱进行色谱分离。第一步，在稀释后的尿液样本中检测到毒蕈碱。第二步，用双分子丹酰化方法发现了麝香酚和伊博藤酸。该方法已成功应用于疑似蘑菇中毒患者尿液样本的常规化学和毒理学分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Analytical Chemistry 化学-分析化学

CiteScore

2.10

自引率

9.10%

发文量

146

审稿时长

13 months

期刊介绍： The Journal of Analytical Chemistry is an international peer reviewed journal that covers theoretical and applied aspects of analytical chemistry; it informs the reader about new achievements in analytical methods, instruments and reagents. Ample space is devoted to problems arising in the analysis of vital media such as water and air. Consideration is given to the detection and determination of metal ions, anions, and various organic substances. The journal welcomes manuscripts from all countries in the English or Russian language.