Evaluation of mechanical transmission of Anaplasma marginale by Stomoxys calcitrans

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-04-16 DOI:10.1016/j.rvsc.2025.105655

Luciana Maffini Heller , Dina María Beltrán Zapa , Igor Maciel Lopes de Morais , Vanessa Ferreira Salvador , Luccas Lourenzzo Lima Lins Leal , Luiz Fellipe Monteiro Couto , Lídia Mendes de Aquino , Lucianne Cardoso Neves , Bianca Barbara Fonseca da Silva , Lorena Lopes Ferreira , Antonio Thadeu Medeiros de Barros , Paulo Henrique Duarte Cançado , Felipe da Silva Krawczak , Caio Marcio de Oliveira Monteiro , Welber Daniel Zanetti Lopes

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Abstract

The objective of this study was to investigate the mechanical transmission of Anaplasma marginale to cattle by Stomoxys calcitrans. The S. calcitrans were obtained in field condition and their offspring (first generation) were used in this study originating in the laboratory. In method 1 (flies released freely in cattle pens), from D0 to D + 10, approximately 3300 flies fasted for 24 h were released daily per pen with infected and healthy calves. In the second method (feeding chambers for artificial interruption of blood meals), for each negative calf, three chambers were used, each containing four flies fasted for 24 h, totaling 12 flies per calf. Blood smears, PCR and ELISA were performed to detect A. marginale in the animals. Furthermore, the animals' head tossing and hoof stomping and the number of flies near the pens' inner walls were recorded. In the presence of the flies, the calves exhibited defensive movements compared to those without flies. None of the negative calves demonstrated the presence of A. marginale (blood smear, PCR, and ELISA). In conclusion, although A. marginale was found on the abdomen of S. calcitrans, this fly failed to transmit this rickettsia to cattle in either transmission method employed.

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.