Novel Approach Using Real-Time Dynamic Imaging Analysis to Monitor Cellular Apoptosis, Viability, and Cell Density in CHO Cell-Based Bioprocesses for Monoclonal Antibody Production

IF 3.2 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Biotechnology Journal Pub Date : 2025-04-14 DOI:10.1002/biot.70018

Claudia Corredor, Yuxiang Zhao, Suyang Wu, James K. Drennen, Carl Anderson

引用次数: 0

Abstract

We investigated an analytical method based on dynamic imaging analysis (DIA) for monitoring cellular apoptosis, viability, and cell density. Trypan blue and flow cytometry were used as reference methods. The DIA method showed results comparable to the traditional methods, with clear advantages (label-free and in-line real-time monitoring capability). The DIA method successfully traced the trajectory of cell death progress, detecting the onset of apoptosis earlier than the other methods.

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利用实时动态成像分析监测单克隆抗体生产中CHO细胞生物过程中细胞凋亡、活力和细胞密度的新方法

我们研究了一种基于动态成像分析（DIA）的分析方法，用于监测细胞凋亡、活力和细胞密度。参照方法为台盼蓝法和流式细胞术。DIA方法的结果与传统方法相当，具有明显的优势（无标签和在线实时监测能力）。DIA方法成功地追踪了细胞死亡进程的轨迹，比其他方法更早地检测到细胞凋亡的发生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Journal Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

8.90

自引率

2.10%

发文量

123

审稿时长

1.5 months

期刊介绍： Biotechnology Journal (2019 Journal Citation Reports: 3.543) is fully comprehensive in its scope and publishes strictly peer-reviewed papers covering novel aspects and methods in all areas of biotechnology. Some issues are devoted to a special topic, providing the latest information on the most crucial areas of research and technological advances. In addition to these special issues, the journal welcomes unsolicited submissions for primary research articles, such as Research Articles, Rapid Communications and Biotech Methods. BTJ also welcomes proposals of Review Articles - please send in a brief outline of the article and the senior author''s CV to the editorial office. BTJ promotes a special emphasis on: Systems Biotechnology Synthetic Biology and Metabolic Engineering Nanobiotechnology and Biomaterials Tissue engineering, Regenerative Medicine and Stem cells Gene Editing, Gene therapy and Immunotherapy Omics technologies Industrial Biotechnology, Biopharmaceuticals and Biocatalysis Bioprocess engineering and Downstream processing Plant Biotechnology Biosafety, Biotech Ethics, Science Communication Methods and Advances.