WNT Signaling Cascade Proteins and Structural and Functional State of The Vascular Bed in Patients With Various Phenotypes of Stable Ischemic Heart Disease.

IF 0.5 4区医学 Q4 CARDIAC & CARDIOVASCULAR SYSTEMS

Kardiologiya Pub Date : 2025-03-31 DOI:10.18087/cardio.2025.3.n2853

A O Iusupova, N N Pakhtusov, O A Slepova, E V Privalova, Yu N Belenkov

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引用次数: 0

Abstract

Aim To evaluate the concentration of the WNT signaling cascade proteins (WNT1, -3a, -4, -5a) and the state of the vasculature by photoplethysmography (PPG) in patients with different phenotypes of stable ischemic heart disease (IHD), with obstructive and non-obstructive coronary artery disease (CAD).Material and methods This cross-sectional observational study included 80 patients (45-75 years old) with a verified diagnosis of stable IHD. Based on the results of coronary angiography or multislice spiral computed tomography coronary angiography, the patients were divided into two equal groups (n=40), with obstructive IHD (oIHD), and ischemia with no obstructive CAD or angina with no obstructive CAD (INOCA/ANOCA). In the oIHD group, men prevailed (67.5%) while in the INOCA/ANOCA group, women prevailed (57.5%). Noninvasive PPG evaluation of the vasculature was performed, and WNT1, -3a, -4, and -5a concentrations were measured by ELISA in all patients.Results Higher concentrations of the WNT1 and WNT3a proteins were found in patients with oIHD (p<0.001) while the INOCA/ANOCA group had a significantly higher concentration of WNT5a (p=0.001). According to the PPG data, the arterial stiffness index (aSI) significantly differed between the INOCA/ANOCA (7.6 m/s [6.6; 9.35]) and oIHD (9.25 m/s [7.88; 10.33]) groups, p=0.048). The correlation analysis revealed a relationship between WNT1 and the reflectance index RI (ρ=0.359; p=0.014) in IHD patients (oIHD+INOCA/ANOCA). According to the ROC analysis, the curve for WNT3a turned out to be diagnostically significant (sensitivity and specificity of the model were 85.7 and 87.0%, respectively). The cut-off value of WNT3a was 0.183 pg/ml.Conclusion The results of the study showed that the activation of the canonical WNT cascade (WNT1 and WNT3a) was observed in patients with oIHD, while the non-canonical cascade (WNT5a) was activated in patients with INOCA/ANOCA. The obstructive IHD phenotype can be predicted with a WNT3a value ≥0.183 pg/ml.

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WNT信号级联蛋白与不同表型稳定型缺血性心脏病患者血管床的结构和功能状态

目的通过光体积描记术（PPG）评价不同表型的稳定型缺血性心脏病（IHD）合并阻塞性和非阻塞性冠状动脉疾病（CAD）患者WNT信号级联蛋白（WNT1, -3a, -4, -5a）的浓度和血管状态。材料和方法本横断面观察性研究包括80例确诊为稳定IHD的患者（45-75岁）。根据冠状动脉造影或多层螺旋ct冠状动脉造影结果，将患者平均分为阻塞性IHD （oIHD）组和缺血无阻塞性CAD或心绞痛无阻塞性CAD （INOCA/ANOCA）组，各40例。在oIHD组中，男性占67.5%，而在INOCA/ANOCA组中，女性占57.5%。对所有患者进行无创PPG血管评估，并通过ELISA检测WNT1、-3a、-4和-5a浓度。结果oIHD患者中wntt1和WNT3a蛋白含量较高（p < 0.001），而INOCA/ANOCA组WNT5a蛋白含量显著高于对照组（p < 0.001）。根据PPG数据，动脉刚度指数（aSI）在INOCA/ANOCA (7.6 m/s；9.35])和oIHD (9.25 m/s [7.88；10.33])组，p=0.048)。相关分析显示WNT1与反射率RI之间存在相关性(ρ=0.359；p=0.014)， IHD患者（oIHD+INOCA/ANOCA）。根据ROC分析，WNT3a的曲线具有诊断意义（模型的敏感性为85.7，特异性为87.0%）。WNT3a的临界值为0.183 pg/ml。结论本研究结果显示，oIHD患者存在典型WNT级联（WNT1和WNT3a）激活，而INOCA/ANOCA患者存在非典型WNT级联（WNT5a）激活。WNT3a值≥0.183 pg/ml可预测阻塞性IHD表型。

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来源期刊

Kardiologiya 医学-心血管系统

CiteScore

1.70

自引率

20.00%

发文量

审稿时长

3-8 weeks

期刊介绍： “Kardiologiya” (Cardiology) is a monthly scientific, peer-reviewed journal committed to both basic cardiovascular medicine and practical aspects of cardiology. As the leader in its field, “Kardiologiya” provides original coverage of recent progress in cardiovascular medicine. We publish state-of-the-art articles integrating clinical and research activities in the fields of basic cardiovascular science and clinical cardiology, with a focus on emerging issues in cardiovascular disease. Our target audience spans a diversity of health care professionals and medical researchers working in cardiovascular medicine and related fields. The principal language of the Journal is Russian, an additional language – English (title, authors’ information, abstract, keywords). “Kardiologiya” is a peer-reviewed scientific journal. All articles are reviewed by scientists, who gained high international prestige in cardiovascular science and clinical cardiology. The Journal is currently cited and indexed in major Abstracting & Indexing databases: Web of Science, Medline and Scopus. The Journal''s primary objectives Contribute to raising the professional level of medical researchers, physicians and academic teachers. Present the results of current research and clinical observations, explore the effectiveness of drug and non-drug treatments of heart disease, inform about new diagnostic techniques; discuss current trends and new advancements in clinical cardiology, contribute to continuing medical education, inform readers about results of Russian and international scientific forums; Further improve the general quality of reviewing and editing of manuscripts submitted for publication; Provide the widest possible dissemination of the published articles, among the global scientific community; Extend distribution and indexing of scientific publications in major Abstracting & Indexing databases.