{"title":"Genetic engineering for SIT application: a fruit fly-focused review.","authors":"Serafima Davydova, Danheng Yu, Angela Meccariello","doi":"10.1111/1744-7917.70038","DOIUrl":null,"url":null,"abstract":"<p><p>Sterile insect technique (SIT) has become a key component of efficient pest control. Fruit fly pests from the Drosophilidae and Tephritidae families pose a substantial and overwhelmingly increasing threat to the agricultural industry, aggravated by climate change and globalization among other contributors. In this review, we discuss the advances in genetic engineering aimed to improve the SIT-mediated fruit fly pest control. This includes SIT enhancement strategies such as novel genetic sexing strain and female lethality approaches. Self-pervasive X-shredding and X-poisoning sex distorters, alongside gene drive varieties are also reviewed. The self-limiting precision-guided SIT, which aims to tackle female removal and male fertility via CRISPR/Cas9, is additionally introduced. By using examples of existing genetic tools in the fruit fly pests of interest, as well as model species, we illustrate that the population control intensity may be modulated depending on strategy selection.</p>","PeriodicalId":13618,"journal":{"name":"Insect Science","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/1744-7917.70038","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Sterile insect technique (SIT) has become a key component of efficient pest control. Fruit fly pests from the Drosophilidae and Tephritidae families pose a substantial and overwhelmingly increasing threat to the agricultural industry, aggravated by climate change and globalization among other contributors. In this review, we discuss the advances in genetic engineering aimed to improve the SIT-mediated fruit fly pest control. This includes SIT enhancement strategies such as novel genetic sexing strain and female lethality approaches. Self-pervasive X-shredding and X-poisoning sex distorters, alongside gene drive varieties are also reviewed. The self-limiting precision-guided SIT, which aims to tackle female removal and male fertility via CRISPR/Cas9, is additionally introduced. By using examples of existing genetic tools in the fruit fly pests of interest, as well as model species, we illustrate that the population control intensity may be modulated depending on strategy selection.
期刊介绍：
Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.
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